PMID: 1191676Nov 18, 1975Paper

Hydrophobic interaction chromatography on non-charged Sepharose derivatives. Binding of a model protein, related to ionic strength, hydrophobicity of the substituent, and degree of substitution (determined by NMR).

Biochimica Et Biophysica Acta
J RosengrenS Hjertén

Abstract

A series of agarose gels, substituted with hydrophobic groups, has been synthesized and used for binding studies with the coloured model protein, phycoerythrin. The degrees of substitution for the derivatives can easily be estimated with proton magnetic resonance (NMR). It has been found that the capacity of the derivatives for phycoerythrin increases with increasing hydrophobicity of the substituent, degree of substitution and increasing ionic strength. For column experiments the degree of substitution should lie in the range 40-100 mmol substituent/mol galactose. When it is excessively high, the flow characteristics of the columns are unsatisfactory and difficulties to achieve complete desorption may arise.

References

Mar 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·S Shaltiel, Z Er-El
Aug 21, 1973·Biochemical and Biophysical Research Communications·B H Hofstee

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Citations

Jan 1, 1992·Archives of Microbiology·Z G ChenU Sankawa
Jan 1, 1978·The International Journal of Biochemistry·R J Yon
Aug 1, 1983·Journal of Steroid Biochemistry·D J Lamb, D W Bullock
Oct 1, 1979·Journal of Biochemical and Biophysical Methods·S HjerténJ Rosengren
Jan 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·C HomcyE Haber
Apr 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·M RigbiJ C Sutherland
Sep 1, 1982·Plant Physiology·H W Siegelman, J H Kycia
Mar 29, 1982·Biochimica Et Biophysica Acta·C Schafer-Nielsen, C Rose
Nov 1, 1983·Biotechnology and Bioengineering·M Nemat-Gorgani, K Karimian
Apr 1, 1985·Biotechnology and Bioengineering·E SadaM Shiozawa
Jun 13, 2012·Journal of Separation Science·Muhammad AasimMarcelo Fernandez-Lahore
Dec 1, 1979·European Journal of Biochemistry·M Dettwiler, K Kirschner
Dec 1, 1989·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·K O ErikssonS Hjertén
Nov 1, 1985·Quarterly Reviews of Biophysics·K D Collins, M W Washabaugh
Mar 10, 1999·Biopolymers·J Y Gao, P L Dubin
Jun 9, 2015·Magnetic Resonance in Chemistry : MRC·Fredrik ElwingerIstván Furó

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