PMID: 1263Dec 1, 1975

Hydrophobic interaction determined by partition in aqueous two-phase systems. Partition of proteins in systems containing fatty-acid esters of poly(ethylene glycol)

European Journal of Biochemistry
V P Shanbhag, C G Axelsson

Abstract

In this report we describe a new method which is useful for measuring hydrophobic interactions between aliphatic hydrocarbon chains and proteins in aqueous environment. The method is based on partition of proteins in an aqueous two-phase system containing dextran and poly(ethylene glycol) and different fatty acid esters of poly(ethylene glycol). The partition is measured under conditions where contributions from electrostatic interactions are eliminated. The difference in partition of proteins in phase systems with and without hyrocarbon groups bound to poly(ethylene glycol), deltalog K, where K is the partition coefficient, is taken as a measure of hydrophobic interaction. Deltalog K varies with size of hydrocarbon chain and type of protein. The length of the aliphatic chain should be greater than 8 carbon atoms in order to get a measurable effect in terms of deltalog K. Bovine serum albumin, beta-lactoglobulin, hemoglobin and myoglobin have been shown to have different affinities for palmitic acid ester of poly(ethylene glycol). No hydrophobic effect could be observed for ovalbumin, cytochrome c or alpha-chymotrypsinogen A.

References

Apr 7, 1975·Biochemical and Biophysical Research Communications·B H Hofstee
Oct 8, 1974·Biochemistry·J SteinhardtK S Birdi
Jan 1, 1974·Acta Chemica Scandinavica. Series B: Organic Chemistry and Biochemistry·G Johansson
Dec 23, 1974·Biochemical and Biophysical Research Communications·V P Shanbhag, G Johansson
Apr 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·R A Capaldi, G Vanderkooi
Mar 1, 1973·European Journal of Biochemistry·G JohanssonP A Albertsson
Jul 1, 1970·Proceedings of the National Academy of Sciences of the United States of America·J A Reynolds, C Tanford
Oct 1, 1969·Journal of Biochemistry·K Ikeda, K Hamaguchi
Dec 15, 1962·Proceedings of the National Academy of Sciences of the United States of America·A WISHNIA
Jan 1, 1959·Advances in Protein Chemistry·W KAUZMANN

Citations

Mar 10, 1999·Biopolymers·J Y Gao, P L Dubin
Jan 1, 1993·Critical Reviews in Food Science and Nutrition·S Nakai, E Li-Chan
Jan 15, 1979·European Journal of Biochemistry·V P Shanbhag, G Johansson
Feb 11, 1999·Bioscience, Biotechnology, and Biochemistry·S AkibaH Kumagai
Jun 16, 2015·Biotechnology and Bioengineering·Sven AmrheinJürgen Hubbuch
Dec 1, 1987·Analytical Biochemistry·D L Sackett, J Wolff
Sep 23, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Bin JiangChunhong Liu
Nov 18, 2018·Interface Focus·Charles D Crowe, Christine D Keating
Jul 1, 1983·Hoppe-Seyler's Zeitschrift für physiologische Chemie·A HinkkanenK Decker

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