Hydrophobic Plasmonic Nanoacorn Array for a Label-Free and Uniform SERS-Based Biomolecular Assay.

ACS Applied Materials & Interfaces
Kai ZhuYiping Cui

Abstract

A surface-enhanced Raman scattering (SERS) aptasensor based on a hydrophobic assembled nanoacorn (HANA) was developed with improved reproducibility and reduced nonspecific binding effect. In the fabrication process, a hexagonal-packed gold film over nanosphere (AuFON) arrays was first obtained and used as a hydrophobic plasmonic substrate. Then, a uniform sub-3 nm molecular spacer array (containing Raman reporters) was prepared by patterning nanometric hydrophilic ultrathin patches onto the hydrophobic AuFON, in which the hydrophilic thin layer is composed of polymers and aptamers. During the sensing process, the HANA aptasensor smartly impedes the adsorption of SERS probes as Au@Ag nanocubes (Au@Ag NCs) in the absence of targets. In the presence of targets, the displacement of aptamers occurs due to the specific interaction between the targets and the aptamers, and the Au@Ag NCs can be assembled onto the hydrophilic patches on AuFON through electrostatic interactions with polymers. Thus, SERS signals of reporter molecules inside the spacer can be dramatically enhanced due to the formation of a nanoparticle-on-mirror (NPoM) array. In such a SERS aptasensor, the well-ordered distribution of SERS probes ensures excellent repeatab...Continue Reading

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