Hydrophobic solvent induced phase transition extraction to extract drugs from plasma for high performance liquid chromatography-mass spectrometric analysis

Journal of Chromatography. a
Guozhu LiuShouzhuo Yao

Abstract

Novel sample preparation approaches for HPLC bioanalysis based on the phenomenon that acetonitrile can be separated from water by adding salts or cooling at subzero temperatures have been reported. These two methods are superior to conventional liquid-liquid extraction since the separated acetonitrile phase can be directly injected to the RP-LC system. However, the salting-out method suffers from a potential problem that the remained salt in the acetonitrile phase may harm the MS detector, while the subzero-temperature method is troublesome to operate. Here, we have reported a similar phase separation phenomenon that the acetonitrile aqueous mixture can be separated by adding a hydrophobic solvent; and capitalising on this phase transition phenomenon, we have proposed an alternative approach, named solvent induced phase transition extraction (SIPTE), to extract drug from plasma for HPLC-MS analysis. The proposed SIPTE method is much simpler and avoids contaminating the MS detector. Three structurally diverse drugs were selected as test compounds to design the SIPTE method and to validate the efficiency of this method. The four goals of plasma sample pretreatment for HPLC-MS analysis, i.e. removal of proteins, removal of other l...Continue Reading

Citations

May 23, 2012·Bioanalysis·Christopher Singleton
Jun 26, 2013·Bioanalysis·Yong Q Tang, Naidong Weng
Aug 1, 2014·Bioanalysis·Somaieh Soltani, Abolghasem Jouyban
Jan 27, 2011·Journal of Separation Science·Guozhu LiuShouzhuo Yao
Dec 31, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Yao ShenTeris A van Beek
Jun 21, 2021·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Fengjie YuXijuan Tu
Sep 3, 2021·Journal of Separation Science·Sherin F HammadFotouh R Mansour

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