Hydrophobic surfactant-associated protein in whole lung surfactant and its importance for biophysical activity in lung surfactant extracts used for replacement therapy

Pediatric Research
J A WhitsettR H Notter

Abstract

Hydrophobic protein of 6,000 and 14,000 daltons was isolated from mammalian pulmonary surfactant obtained from canine, human, and bovine alveolar lavage material. Low molecular weight, hydrophobic, surfactant-associated protein (SAP), herein referred to as SAP 6-14, was distinguished from SAP-35, the major glycoprotein in mammalian surfactants (the 35,000 dalton glycoprotein A or apolipoprotein A) by amino acid composition, peptide mapping, and by resistance of SAP 6-14 to digestion by endoglycosidase F, collagenase, trypsin, and other proteases. The amino acid composition of SAP 6-14 was found to be highly enriched in leucine and other hydrophobic amino acids. The characteristics of protein isolated from bovine replacement surfactant extracts utilized for the treatment of hyaline membrane disease in humans were also studied. SAP 6-14 isolated from calf lung surfactant replacement extracts (CLSE) and surfactant-TA were found to be identical to SAP 6-14 isolated from ether/ethanol extracts of various mammalian surfactants. By contrast, SAP-35, the major surfactant-associated glycoprotein of molecular weight = 35,000, and other higher molecular weight proteins were not detected in significant quantities in the CLSE or surfactant-...Continue Reading

Citations

May 1, 1996·Pediatric Pulmonology·D A EvansJ A Whitsett
Oct 17, 1987·Biochimica Et Biophysica Acta·S H YuF Possmayer
Mar 14, 1989·Biochimica Et Biophysica Acta·J M SnyderJ A O'Brien
Nov 6, 1989·Biochimica Et Biophysica Acta·A BaritussioG Crepaldi
Apr 26, 1989·Biochimica Et Biophysica Acta·J ChungF Possmayer
May 1, 1990·Biochimica Et Biophysica Acta·A TakahashiH W Taeusch
Nov 1, 1988·Chemistry and Physics of Lipids·B D Fleming, K M Keough
Jan 1, 1991·Chemistry and Physics of Lipids·A R VenkitaramanR H Notter
May 22, 1995·Chemistry and Physics of Lipids·A D Horowitz
Mar 31, 1995·Clinica Chimica Acta; International Journal of Clinical Chemistry·J TaïebJ Bourbon
Aug 17, 1990·Biochimica Et Biophysica Acta·S MatalonB A Freeman
Jan 1, 1988·General Pharmacology·T E Weaver
Jun 1, 1990·Baillière's Clinical Endocrinology and Metabolism·C R Mendelson, V Boggaram
Feb 11, 1993·The New England Journal of Medicine·L M NogeeH R Colten
Feb 24, 2001·The New England Journal of Medicine·L M NogeeJ A Whitsett
May 10, 2001·Proceedings of the National Academy of Sciences of the United States of America·S W GlasserJ A Whitsett
Jan 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·S HawgoodR T White
Jun 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·S W GlasserJ A Whitsett
Nov 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·R G WarrR T White
Dec 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·H G LileyP L Ballard
Apr 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·V K SarinJ L Fox
Mar 1, 2010·Pediatric Allergy, Immunology, and Pulmonology·Lawrence M Nogee
May 25, 2006·Current Opinion in Pediatrics·Lawrence M Nogee
Jan 1, 1991·Acta Anaesthesiologica Scandinavica. Supplementum·B Robertson
Jan 1, 1997·Annual Review of Physiology·J A Clements

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.