Hydrophobin Vmh2-glucose complexes self-assemble in nanometric biofilms.

Journal of the Royal Society, Interface
Ilaria ReaLuca De Stefano

Abstract

Hydrophobins are small proteins secreted by fungi, which self-assemble into amphipathic membranes at air-liquid or liquid-solid interfaces. The physical and chemical properties of some hydrophobins, both in solution and as a biofilm, are affected by poly or oligosaccharides. We have studied the interaction between glucose and the hydrophobin Vmh2 from Pleurotus ostreatus by spectroscopic ellipsometry (SE), atomic force microscopy (AFM) and water contact angle (WCA). We have found that Vmh2-glucose complexes forms a chemically stable biofilm, obtained by drop deposition on silicon, 1.6 nm thick and containing 35 per cent of glucose, quantified by SE. AFM highlighted the presence of nanometric rodlet-like aggregates (average height, width and length being equal to 3.6, 23.8 and 40 nm, respectively) on the biofilm surface, slightly different from those obtained in the absence of glucose (4.11, 23.9 and 64 nm). The wettability of a silicon surface, covered by the organic layer of Vmh2-glucose, strongly changed: WCA decreased from 90° down to 17°.

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Citations

Sep 25, 2015·Colloids and Surfaces. B, Biointerfaces·Jane PolitiJolanda Spadavecchia
Jul 26, 2015·The Protein Journal·Mohammadreza KhalesiGuy Derdelinckx
Jan 8, 2015·Applied Microbiology and Biotechnology·Han A B Wösten, Karin Scholtmeijer
Jun 3, 2016·Journal of Colloid and Interface Science·Olivier EmileAziz Ghoufi
Jul 21, 2016·Biotechnology for Biofuels·Neil Andrew BrownGustavo H Goldman

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