Hydrosalpinx fluid affects murine embryonic development in a coculture system with epithelial endometrial cells

Fertility and Sterility
I CarrascoD Vantman

Abstract

The aim of the present investigation was to assess whether a coculture system protects from the effect of hydrosalpinx fluid (HF) on murine embryo development, evaluated through blastocyst cell number. Controlled prospective study. Academic research center. Endometrium and HF from six patients and endometrium from six normal patients. Murine embryos were exposed to the absence or presence of different concentrations of human HF: 0% HF (control), 50% HF, 70% HF in human tubal fluid, and 100% HF, in a simple culture system (SCS), epithelial coculture system (ECS), and hydrosalpinx epithelial coculture system (HECS). Embryonic development at 72 hours and blastocyst cell number determined by the Tarcowsky method. In SCS, 91.9% of the embryos reached the blastocyst stage, and no significant differences were shown in the presence of HF. However, significant differences were observed in the blastocyst cell number. Of the embryos cultured in ECS, 97.1% reached the blastocyst stage, and high concentrations of HF caused a decrease in embryonic development. A significant difference was observed between ECS and HECS in embryo development without HF. When HF was added, a significant decrease in blastocyst cell number was seen in embryos exp...Continue Reading

References

Jun 1, 1991·Journal of in Vitro Fertilization and Embryo Transfer : IVF·R T MansourR Riad
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Apr 30, 1999·Human Reproduction·M A CohenM V Sauer

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Citations

May 2, 2012·Journal of Occupational Medicine and Toxicology·Myeong-Seop LeeHo-Yeon Song
Feb 12, 2005·Human Reproduction·Louis Chukwuemeka AjonumaHsiao Chang Chan

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