Hydrostatic and osmotic pressure study of the hairpin ribozyme

Biochimica Et Biophysica Acta
Guy HervéMarie-Christine Maurel

Abstract

The recent discovery of numerous catalytically active RNAs in various living species as well as the in vitro selection of a large series of RNA aptamers able to bind specifically various molecules such as metabolites and co-factors, emphasize the adaptability of RNAs through the plasticity of their secondary structure. Furthermore, all these observations give support to the "RNA world" hypothesis as a step in the primitive development of life on Earth. On this background, we used high pressure to study the mechanism of action of a model hairpin ribozyme which exhibits self-cleavage and ligation. The activation volume (DeltaV( not equal)) of the cleavage reaction (34+/-4 ml/mol) indicates that an important compaction of the RNA molecule occurs during the reaction and must be accompanied by a significant movement of water molecules . Indeed, such a release of 78+/-4 water molecules per RNA molecule could be measured by complementary osmotic shock experiments. These results are consistent with the information provided by the structural studies which indicate that two loops of the RNA molecule should come into contact for the reaction to occur . The high pressure study of a modified form of the ribozyme whose activity is strictly d...Continue Reading

References

Oct 14, 1988·Biochemical and Biophysical Research Communications·J M BuzayanF Eckstein
Dec 1, 1965·Proceedings of the National Academy of Sciences of the United States of America·C R Woese
Dec 1, 1968·Journal of Molecular Biology·L E Orgel
Feb 1, 1983·Quarterly Reviews of Biophysics·G Weber, H G Drickamer
Jan 1, 1982·Annual Review of Biophysics and Bioengineering·K Heremans
Dec 15, 1995·The Journal of Biological Chemistry·S E ButcherJ M Burke
Oct 10, 1997·Nucleic Acids Research·K J YoungJ A Grasby
Apr 3, 2001·Science·M M YusupovH F Noller
Mar 9, 2002·Nucleic Acids Research·Sean P Ryder, Scott A Strobel
May 23, 2002·RNA·Timothy J Wilson, David M J Lilley
Jul 12, 2002·Nature·Gerald F Joyce
Jul 12, 2002·Nature·Jennifer A Doudna, Thomas R Cech
Aug 20, 2002·Proceedings of the National Academy of Sciences of the United States of America·K Mark ParnellScott A Strobel
Nov 21, 2002·Biochemical Society Transactions·M J Fedor
Jan 10, 2003·The Journal of Biological Chemistry·Marc MeliMarie-Christine Maurel
Jul 29, 2003·Proceedings of the National Academy of Sciences of the United States of America·Elliot TanTaekjip Ha
May 5, 2005·Nucleic Acids Research·Sylvia TobéMarie-Christine Maurel
Aug 3, 2005·Proceedings of the National Academy of Sciences of the United States of America·Sidney AltmanYong Li

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Citations

Jul 10, 2007·Nucleic Acids Research·Eric GirardRoger Fourme
Sep 24, 2015·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Trung Quan LuongRoland Winter
Nov 14, 2014·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Caroline SchuabbRoland Winter
Jul 8, 2015·Chembiochem : a European Journal of Chemical Biology·Shu-ichi NakanoNaoki Sugimoto
Mar 31, 2017·Nature Communications·Caroline SchuabbRoland Winter
Aug 2, 2018·Physical Chemistry Chemical Physics : PCCP·Narendra Kumar, Dominik Marx
Nov 13, 2019·Chemistry, an Asian Journal·Kristian Le VayHannes Mutschler
Feb 11, 2020·Nucleosides, Nucleotides & Nucleic Acids·Md Sohanur RahmanYoshiya Ikawa
Nov 7, 2018·Proceedings of the National Academy of Sciences of the United States of America·Bishnu P PaudelDavid S Rueda
Mar 6, 2013·Biochemistry·Mary Jane TimsonElizabeth E Howell
Oct 22, 2020·The Journal of Physical Chemistry Letters·Narendra Kumar, Dominik Marx

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