Hydrostatic Pressure Helps to Cultivate an Original Anaerobic Bacterium From the Atlantis Massif Subseafloor (IODP Expedition 357): Petrocella atlantisensis gen. nov. sp. nov

Frontiers in Microbiology
Marianne QuéméneurAnne Postec

Abstract

Rock-hosted subseafloor habitats are very challenging for life, and current knowledge about microorganisms inhabiting such lithic environments is still limited. This study explored the cultivable microbial diversity in anaerobic enrichment cultures from cores recovered during the International Ocean Discovery Program (IODP) Expedition 357 from the Atlantis Massif (Mid-Atlantic Ridge, 30°N). 16S rRNA gene survey of enrichment cultures grown at 10-25°C and pH 8.5 showed that Firmicutes and Proteobacteria were generally dominant. However, cultivable microbial diversity significantly differed depending on incubation at atmospheric pressure (0.1 MPa), or hydrostatic pressures (HP) mimicking the in situ pressure conditions (8.2 or 14.0 MPa). An original, strictly anaerobic bacterium designated 70B-AT was isolated from core M0070C-3R1 (1150 meter below sea level; 3.5 m below seafloor) only from cultures performed at 14.0 MPa. This strain named Petrocella atlantisensis is a novel species of a new genus within the newly described family Vallitaleaceae (order Clostridiales, phylum Firmicutes). It is a mesophilic, moderately halotolerant and piezophilic chemoorganotroph, able to grow by fermentation of carbohydrates and proteinaceous comp...Continue Reading

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Citations

Mar 30, 2020·Applied and Environmental Microbiology·Shahrzad MotamediWilliam J Brazelton
Nov 7, 2019·International Journal of Systematic and Evolutionary Microbiology·Aharon Oren, George Garrity
Oct 3, 2020·Environmental Microbiology Reports·Hai-Nan Su, Yu-Zhong Zhang
Apr 24, 2021·Extremophiles : Life Under Extreme Conditions·Anastasia FrolovaA I Slobodkin
May 19, 2021·International Journal of Systematic and Evolutionary Microbiology·Marianne QuéméneurAnne Postec
Dec 6, 2019·Chemical Reviews·Marie-Christine MaurelGuy Hervé

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Datasets Mentioned

BETA
PRJNA448822
KY969626
PRJEB28585
1
LR130778
SRP148750

Methods Mentioned

BETA
PCR
transmission electron microscopy
phosphotransferase

Software Mentioned

R
Excel
Heatmap
QIIME
MEGA7
PEAR
vsearch
XLSTAT
MUSCLE
Uclust

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