Hydrostatic pressure induces apoptosis of chondrocytes cultured in alginate beads

Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society
Shu NakamuraToshikazu Kubo

Abstract

The purpose of this study was to investigate the influence of hydrostatic pressure (HP) on apoptosis and expression of heat-shock protein 70 (HSP70) in chondrocytes cultured in alginate beads. Chondrocytes were isolated from the articular cartilage of rabbit joints and seeded in alginate beads. The beads in Group A were cultured for less than 24 h after being embedded with the chondrocytes, while those in Group B were cultured for 2 weeks. Both groups were exposed to HP of 10 or 50 MPa for 12 or 24 h. The beads in Groups A and B that were not exposed to HP were regarded as controls. Apoptotic cells induced by exposure to HP were quantified using the TUNEL method. Immunohistochemical analysis for HSP70 and in situ TUNEL analysis were also performed. Apoptotic chondrocytes were not observed in the control cells under atmospheric pressure, whereas apoptosis was observed in the beads in Group A, and the number of apoptotic cells increased as the duration and magnitude of HP increased. On the other hand, we observed no significant population of apoptotic cells in the beads in Group B. Chondrocytes expressing HSP70 were not TUNEL positive in the histological analysis. Excessively strong HP could evoke apoptosis when the extracellular...Continue Reading

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Citations

Dec 10, 2009·Journal of Orthopaedic Science : Official Journal of the Japanese Orthopaedic Association·Taisuke KunitomoToshikazu Kubo
Jun 16, 2010·Biomechanics and Modeling in Mechanobiology·Manuela T RaimondiMatteo Moretti
Jan 12, 2012·Biomechanics and Modeling in Mechanobiology·E K MooS Federico
May 9, 2012·Tissue Engineering. Part a·Cristina CorreiaRui L Reis
Feb 7, 2009·Tissue Engineering. Part B, Reviews·Benjamin D Elder, Kyriacos A Athanasiou
Jan 9, 2015·International Journal of Molecular Sciences·Hiroaki InoueToshikazu Kubo
Jun 27, 2018·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Ashley HeuijerjansCorrinus C van Donkelaar
Sep 1, 2009·Tissue Engineering. Part C, Methods·Minggan LiXiongbiao Chen
Feb 24, 2011·Clinical Orthopaedics and Related Research·Sibylle GradMartin J Stoddart
Nov 13, 2009·International Journal of Hyperthermia : the Official Journal of European Society for Hyperthermic Oncology, North American Hyperthermia Group·Kenji A TakahashiToshikazu Kubo
Jul 9, 2008·The International Journal of Artificial Organs·G CandianiG Dubini
Nov 14, 2019·Cellular and Molecular Bioengineering·Pengwei QuGuozhu Wang
Oct 21, 2020·Scientific Reports·Tien Minh LeKenji Kusumoto
Jan 27, 2021·Connective Tissue Research·Alireza SavadipourFarshid Guilak
Dec 20, 2020·International Journal of Molecular Sciences·Markus WehlandDaniela Grimm

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Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis