PMID: 9442112Feb 28, 1998Paper

Hydrostatic pressure shows that lamellipodial motility in Ascaris sperm requires membrane-associated major sperm protein filament nucleation and elongation

The Journal of Cell Biology
T M RobertsM Stewart

Abstract

Sperm from nematodes use a major sperm protein (MSP) cytoskeleton in place of an actin cytoskeleton to drive their ameboid locomotion. Motility is coupled to the assembly of MSP fibers near the leading edge of the pseudopod plasma membrane. This unique motility system has been reconstituted in vitro in cell-free extracts of sperm from Ascaris suum: inside-out vesicles derived from the plasma membrane trigger assembly of meshworks of MSP filaments, called fibers, that push the vesicle forward as they grow (Italiano, J.E., Jr., T.M. Roberts, M. Stewart, and C.A. Fontana. 1996. Cell. 84:105-114). We used changes in hydrostatic pressure within a microscope optical chamber to investigate the mechanism of assembly of the motile apparatus. The effects of pressure on the MSP cytoskeleton in vivo and in vitro were similar: pressures >50 atm slowed and >300 atm stopped fiber growth. We focused on the in vitro system to show that filament assembly occurs in the immediate vicinity of the vesicle. At 300 atm, fibers were stable, but vesicles often detached from the ends of fibers. When the pressure was dropped, normal fiber growth occurred from detached vesicles but the ends of fibers without vesicles did not grow. Below 300 atm, pressure m...Continue Reading

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Citations

Oct 1, 1990·Immunology Today·R J Noelle, E C Snow
Mar 21, 1998·Nature Structural Biology·T L BullockM Stewart
Apr 4, 2008·Proceedings of the National Academy of Sciences of the United States of America·Long MiaoThomas M Roberts
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Oct 24, 2006·Biophysical Journal·Richard B Dickinson, Daniel L Purich
Mar 10, 2005·Journal of Molecular Biology·Richard P GrantMurray Stewart
Jan 30, 2008·Biophysical Journal·Mark ZajacCharles W Wolgemuth
Sep 19, 2003·Current Biology : CB·Alex Mogilner, George Oster
Nov 25, 2003·Science·Long MiaoThomas M Roberts
May 7, 2003·Physical Review Letters·Jean-François JoannyJacques Prost

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