PMID: 9187403Apr 25, 1997Paper

Hydroxyindole-O-methyltransferase activity assay using high-performance liquid chromatography with fluorometric detection: determination of melatonin enzymatically formed from N-acetylserotonin and S-adenosyl-L-methionine

Journal of Chromatography. B, Biomedical Sciences and Applications
M T ItohY Sumi

Abstract

A reliable, sensitive and rapid assay has been developed for determining the activity of hydroxyindole-O-methyltransferase (HIOMT; S-adenosyl-L-methionine:N-acetylserotonin-O-methyltransferase; EC 2.1.1.4), which catalyzes the final step in the melatonin (N-acetyl-5-methoxytryptamine) biosynthetic pathway. This method is based on the separation and detection of melatonin formed enzymatically from N-acetylserotonin and S-adenosyl-L-methionine, by high-performance liquid chromatography with fluorometric detection. The detection limit for melatonin formed per sample was as low as 150 fmol, indicating that the sensitivity of this assay was comparable to that of a radioisotopic assay. The assay was applied to the determination of HIOMT activity in rat pineal gland. The HIOMT activity obtained in this study was comparable with, or slightly lower than those reported previously using radioisotopic assays.

References

Jun 28, 1974·Science·J Axelrod
Jan 1, 1973·Neuroendocrinology·D P CardinaliR J Wurtman
Apr 29, 1960·Science·J AXELROD, H WEISSBACH

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Citations

Feb 1, 2002·FEBS Letters·Andrzej SlominskiJacobo Wortsman
Dec 11, 2007·International Journal of Toxicology·Elena B C LlambíasLeonor C San Martín de Viale
Apr 7, 1999·Biomedical Chromatography : BMC·H Y Aboul-EneinA Covaci
Jun 1, 2002·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Andrzej SlominskiOlle Johansson
Dec 30, 2020·International Journal of Molecular Sciences·Kamila MartyniukBogdan Lewczuk
Nov 30, 2021·Archiv der Pharmazie·Hussain AlessaJalal T Althakafy

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