Hydroxyl radical mediates N epsilon-(carboxymethyl)lysine formation from Amadori product.

Biochemical and Biophysical Research Communications
Ryoji NagaiS Horiuchi

Abstract

Recent studies demonstrated N epsilon-(carboxymethyl)lysine (CML) in several tissue proteins. Incubation of proteins with glucose leads through a Schiff base to Amadori products. Oxidative cleavage of Amadori products is considered as a major route to CML formation in vivo, whereas it is not known which reactive oxygen species (ROS) is involved. The present study is undertaken to identify such a ROS. We prepared heavily glycated human serum albumin (HSA) which contained a high level of Amadori products, but an undetectable level of CML. Incubation of glycated HSA with FeCl2, but not with H2O2, led to CML formation which was enhanced by H2O2, but inhibited by catalase or mannitol, whereas superoxide dismutase had no effect. Similar data were obtained by experiments using Boc-fructose-lysine as a model Amadori compound. These data indicate that hydroxyl radical generated by the reaction of Fe2+ with H2O2 mediates CML formation from Amadori compounds.

References

Dec 31, 1990·Biochemical and Biophysical Research Communications·C J MullarkeyM Brownlee
Nov 21, 1995·Biochemistry·M C Wells-KnechtJ W Baynes

❮ Previous
Next ❯

Citations

Jan 25, 2005·Current Diabetes Reports·Peter NawrothDavid M Stern
Jun 21, 2005·Annals of the New York Academy of Sciences·Chun-Jen ChangShih-Yi Huang
Jan 15, 2008·Journal of Clinical Biochemistry and Nutrition·Khaled A AhmedIkram S Ismail
Nov 15, 2007·The Journals of Gerontology. Series A, Biological Sciences and Medical Sciences·LeAnn M SnowLaDora V Thompson
Oct 20, 2010·Amino Acids·Teruo MiyazawaRyoji Nagai
Feb 26, 2016·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Hossam M AbdallahZainy M Banjar
Oct 6, 2017·Journal of Nutritional Science and Vitaminology·Shiori MatsudaRyoji Nagai
Nov 1, 2010·Expert Review of Endocrinology & Metabolism·Katrien Hj GaensCasper G Schalkwijk
Oct 9, 2019·The Journal of Biological Chemistry·Rei-Ichi OhnoRyoji Nagai
Nov 3, 1998·Journal of Clinical Pathology·T MatsuseY Ouchi
Dec 15, 2007·Annals of the New York Academy of Sciences·Ryoji NagaiTsuyoshi Ikeda
Feb 24, 2011·Biomedit︠s︡inskai︠a︡ khimii︠a︡·N A Ansari, Z Rasheed
May 7, 2013·Journal of Food Protection·Lipeng HanGuoqin Liu
Feb 24, 2015·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Seoungwoo ShinDeokhoon Park
Nov 29, 2013·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Weerachat SompongSirichai Adisakwattana
Jun 22, 2016·Glycoconjugate Journal·Naoyuki TaniguchiAkiko Furuta
Jun 21, 2016·Epigenomics·Sarah Kreuz, Wolfgang Fischle
Mar 5, 2019·Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan·Hikari SugawaRyoji Nagai
Apr 4, 2001·The Journal of Clinical Investigation·V M Monnier
Jun 18, 2002·Laboratory Investigation; a Journal of Technical Methods and Pathology·Cristina Miki HayashiSeikoh Horiuchi
Apr 13, 2005·Neuropathology : Official Journal of the Japanese Society of Neuropathology·Motoko KawaguchiMakio Kobayashi
Feb 28, 2004·Experimental Biology and Medicine·Mohammad Asghar, Mustafa F Lokhandwala
May 23, 2007·Free Radical Research·Katsumi MeraMasaki Otagiri
Feb 28, 2007·British Journal of Cancer·A M E BruynzeelW J F van der Vijgh
Jul 24, 2010·Annals of the New York Academy of Sciences·Mei-Chen LoShu-Huei Kao
Oct 8, 2011·Journal of Clinical Biochemistry and Nutrition·Shinya ToyokuniAkira Kawada
Apr 8, 2014·Journal of Molecular Modeling·Christian Solís-CaleroFrancisco Muñoz
Jun 10, 2011·American Journal of Nephrology·Melinda T Coughlan, Josephine M Forbes
Apr 9, 2014·BMC Complementary and Alternative Medicine·Sirichai AdisakwattanaCharoonsri Chusak
Aug 25, 2020·Evidence-based Complementary and Alternative Medicine : ECAM·Nur Sumirah Mohd DomMuhajir Hamid

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.