Hydroxyl radicals are not involved in NADPH dependent microsomal lipid peroxidation

Experientia
A Bast, M H Steeghs

Abstract

NADPH dependent H2O2 formation in microsomes in the presence of chelated iron leads to formation of hydroxyl radicals. Enhancement of hydroxyl radical generation (via ferric-EDTA or sodium azide) did not result in a concomitant increase in lipid peroxidation; rather, a decrease was observed. Moreover, the hydroxyl radical scavenger DMSO did not inhibit lipid peroxidation. This comparison of hydroxyl radical formation with lipid peroxidation suggests that hydroxyl radicals do not play a part in NADPH-dependent lipid peroxidation.

Citations

Jan 1, 1988·The International Journal of Biochemistry·J Z Byczkowski, T Gessner
Jan 1, 1989·The International Journal of Biochemistry·J Z Byczkowski, A P Kulkarni
May 7, 1993·Biochimica Et Biophysica Acta·S Puntarulo, A I Cederbaum
Jan 1, 1989·Free Radical Biology & Medicine·S PuntaruloA I Cederbaum
Sep 15, 1995·European Journal of Pharmacology·G J WesterveldA Bast
Mar 22, 2002·Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan·Tadashi Fujita
Jan 1, 1988·Pharmacological Research Communications·F Shridi, J Robak
Mar 1, 1988·Biochemical Pharmacology·J Robak, R J Gryglewski

Related Concepts

Fe(III)-EDTA, sodium salt, trihydrate
Metazoa
Azides
Rheumabene
Edetic Acid, Calcium, Sodium Salt
Ferric Compounds
Hydroxides
Lipid Peroxides
Microsomes, Liver
NADP

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