Hydroxylamine is a vasorelaxant and a possible intermediate in the oxidative conversion of L-arginine to nitric oxide

Biochemical and Biophysical Research Communications
E G DeMasterE K Weir

Abstract

Our objective was to determine whether hydroxylamine is a possible intermediate in the oxidative conversion of L-arginine to nitric oxide. Vasorelaxation by hydroxylamine is known to be mediated by nitric oxide. The vasorelaxant properties of hydroxylamine were examined using rat aortic rings and an isolated rat lung perfusion model. Hydroxylamine and acetylcholine were equally effective in relaxing norepinephrine-contracted intact aortic rings, whereas only hydroxylamine relaxed aortic rings with endothelium removed. This endothelium-independent vasorelaxation by hydroxylamine indicated that the hydroxylamine-converting enzyme is not localized solely within endothelial cells. Catalase, an enzyme known to oxidize hydroxylamine to nitric oxide, was present in homogenates of intact and endothelium-denuded rings. Cyanamide, another catalase substrate and a known precursor of nitroxyl (HNO), was not a vasorelaxant of aortic rings or of isolated, hypoxia-constricted lungs. These results suggest that free nitroxyl is not an intermediate in the oxidation of hydroxylamine to nitric oxide. An overall pathway for the oxidative conversion of L-arginine through an hydroxylamine intermediate to nitric oxide is proposed.

References

Oct 4, 1976·Biochemical and Biophysical Research Communications·N MikiK Kuriyama
Sep 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·R IyengarM A Marletta
Jan 16, 1989·Biochemical and Biophysical Research Communications·R M Palmer, S Moncada
Jan 1, 1989·Journal of Applied Physiology·S L ArcherE K Weir
Sep 1, 1988·Chemical Research in Toxicology·M A Marletta
Jun 30, 1988·Biochemical and Biophysical Research Communications·R M PalmerS Moncada
Jul 1, 1986·Biochemical Pharmacology·E G DeMasterH T Nagasawa
Oct 1, 1985·Analytical Biochemistry·P K SmithD C Klenk
Aug 1, 1958·Biochimica Et Biophysica Acta·D KEILIN, P NICHOLLS

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Citations

Jun 3, 1996·European Journal of Pharmacology·M N Abdul-HussainS N Hussain
Oct 17, 1996·European Journal of Pharmacology·M H AntoineP Lebrun
Jan 16, 2002·European Journal of Pharmacology·Mohammad ShahidullahWilliam Martin
May 14, 2003·Neurochemistry International·Pirjo Saransaari, Simo S Oja
Jun 17, 2000·Progress in Neurobiology·S S Oja, P Saransaari
Nov 21, 1997·Biochimica Et Biophysica Acta·J TairaP Riesz
Dec 1, 1996·The International Journal of Biochemistry & Cell Biology·P VetrovskyG Entlicher
Dec 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·M E Murphy, H Sies
Jan 18, 2011·Antioxidants & Redox Signaling·Wilmarie Flores-SantanaDavid A Wink
Apr 10, 2009·Journal of Experimental Botany·Stefan RümerWerner M Kaiser
Aug 1, 1995·Journal of Autonomic Pharmacology·J Marín, M A Rodríguez-Martínez
Sep 28, 2010·Neurochemical Research·Pirjo Saransaari, Simo S Oja
Jan 14, 2004·British Journal of Pharmacology·Caroline FradettePatrick Du Souich
Mar 16, 2013·British Journal of Pharmacology·Antonia AsimakopoulouAndreas Papapetropoulos
Mar 17, 2006·Free Radical Biology & Medicine·Sonia DonzelliDavid A Wink
Jun 21, 2005·European Journal of Pharmacology·Petra BeranovaJean-Claude Stoclet
Oct 23, 2009·Plant Signaling & Behavior·Stefan RümerWerner M Kaiser
Feb 11, 2015·Annual Review of Physiology·Nadzeya V Marozkina, Benjamin Gaston
Nov 1, 1993·Angiology·E C SantoianP W Ramwell
Sep 9, 2017·Chemical Communications : Chem Comm·Adam C SedgwickTony D James
Feb 20, 2016·Experimental and Therapeutic Medicine·Ji-Yan LinXiao-Xing Liao
Jan 1, 2017·Metabolomics : Official Journal of the Metabolomic Society·Mona Elbadawi-SidhuRima Kaddurah-Daouk
Apr 11, 2002·Chemical Reviews·Peng George WangAdam J Janczuk
Aug 17, 2010·The Journal of Experimental Biology·Julien M ClaesJérôme Mallefet

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