Hydroxylase regulation in Candida tropicalis grown on alkanes

Canadian Journal of Microbiology
M GilewiczE Azoulay

Abstract

Candida tropicalis synthesizes a hydroxylase (3 to 5 nmol of product formed per minute per milligram of protein) and a cytochrome P-450 (0.10 to 0.13 nmol per milligram of protein) during growth on n-tetradecane. A three- to four-fold increase in the level of NADPH cytochrome c reductase is also observed in those cells as compared to the level of cells grown on glycerol. The most efficient inducers of the hydroxylase and of cytochrome P-450 are straight-chain alkanes having at least 10 carbon atoms. Alkenes and higher alcohols are also good inducers. There is little or no growth on ramified hydrocarbons such as pristane and on long-chain aldehydes and fatty acids. The partial inhibition of growth on decane is probably due to the denaturation of the microsomal electron carrier systems by the fatty acid formed by hydroxylation of the decane in the yeast.

Citations

Nov 1, 1982·Archives of Environmental Contamination and Toxicology·S O KäremlampiO O Hänninen
Jun 16, 1980·Biochemical and Biophysical Research Communications·J C BertrandE Azoulay
Jan 1, 1981·Zeitschrift für allgemeine Mikrobiologie·S MauersbergerH H Müller

Related Concepts

Alkanes
Candida utilis
Cell-Free System
Cytochrome P-450 Oxygenase
Enzyme Induction
Saturated Fat
Glycerin
Mixed Function Oxygenases
Microsomes
NADPH-Ferrihemoprotein Reductase

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