PMID: 2492106Jan 1, 1989Paper

Hydroxylation of aspartic acid in domains homologous to the epidermal growth factor precursor is catalyzed by a 2-oxoglutarate-dependent dioxygenase

Proceedings of the National Academy of Sciences of the United States of America
J StenfloR B Merrifield

Abstract

3-Hydroxyaspartic acid and 3-hydroxyasparagine are two rare amino acids that are present in domains homologous to the epidermal growth factor precursor in vitamin K-dependent plasma proteins as well as in proteins that do not require vitamin K for normal biosynthesis. They are formed by posttranslational hydroxylation of aspartic acid and asparagine, respectively. The first epidermal growth factor-like domain in factor IX (residues 45-87) was synthesized with aspartic acid in position 64, replacing 3-hydroxyaspartic acid. It was used as substrate in a hydroxylase assay with rat liver microsomes as the source of enzyme and reaction conditions that satisfy the requirements of 2-oxoglutarate-dependent dioxygenases. The synthetic peptide stimulated the 2-oxoglutarate decarboxylation in contrast to synthetic, modified epidermal growth factor (Met-21 and His-22 deleted and Glu-24 replaced by Asp) and synthetic peptides corresponding to residues 60-71 in human factor IX. This indicates that the hydroxylase is a 2-oxoglutarate-dependent dioxygenase with a selective substrate requirement.

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Citations

Jan 1, 1992·The International Journal of Biochemistry·K K Han, A Martinage
Aug 29, 2001·Phytochemistry·S MartensR Lukacin
May 22, 2013·International Journal of Cell Biology·Xiaotian Zhong, Jill F Wright
Feb 16, 2007·Nature Chemical Biology·Abdullah Ozer, Richard K Bruick
Jun 6, 2015·Molecular Cell·Athanasios Ploumakis, Mathew L Coleman
Sep 29, 2006·Journal of Thrombosis and Haemostasis : JTH·J Stenflo
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Mar 4, 2006·Journal of Inorganic Biochemistry·Ian J CliftonChristopher J Schofield
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Feb 29, 2020·The Journal of Biological Chemistry·Lennart BrewitzChristopher J Schofield
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Aug 20, 2020·Journal of Experimental & Clinical Cancer Research : CR·Madiha KanwalRuth Tachezy
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Apr 23, 2021·Angewandte Chemie·Amelia BrasnettChristopher J Schofield
Jul 6, 2021·Journal of Fluorine Chemistry·Lennart BrewitzChristopher J Schofield

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