PMID: 235Jul 1, 1975

Hydroxylation of p-Coumaric acid by illuminated chloroplasts. The role of superoxide

European Journal of Biochemistry
B Halliwell

Abstract

1. Chloroplasts isolated from leaves of spinach-beet (Beta vulgaris L. ssp. vulgaris) do not catalyse the hydroxylation of p-coumaric acid in the dark unless a reductant (such as ascorbate, NADH or NADPH) is added. Superoxide dismutase has no effect on this reaction. 2. Illuminated chloroplasts catalyse the hydroxylation in the absence of added reductant. This reaction is completely inhibited by superoxide dismutase, but catalase has little effect. 3. Both hydroxylation in the light and hydroxylation in the dark in the presence of reductants are inhibited by diethyldithiocarbamate, EDTA, cyanide and 2-mercaptoethanol. 4. It is proposed that O-2- generated by illuminated chloroplasts is involved in the provision of a reductant to the enzyme phenolase.

References

Oct 15, 1973·Biochemical and Biophysical Research Communications·S W MayA Felix
Sep 5, 1972·Biochemical and Biophysical Research Communications·R P KumarN A Rao
Oct 1, 1973·The Biochemical Journal·B Halliwell
Feb 1, 1974·The Biochemical Journal·B Halliwell, V S Butt
May 1, 1974·Proceedings of the Society for Experimental Biology and Medicine·T Z LiuW F Ganong
May 7, 1974·Biochemical and Biophysical Research Communications·J Lumsden, D O Hall
Sep 1, 1974·Archives of Biochemistry and Biophysics·J N Siedow, A S Pietro
Jan 31, 1972·Biochemical and Biophysical Research Communications·M NishikimiK Yagi
Feb 1, 1972·Zeitschrift Für Naturforschung. Teil B. Anorganische Chemie, Organische Chemie, Biochemie, Biophysik, Biologie·W Oettmeier, A Heupel
Sep 1, 1974·FEBS Letters·E F Elstner, J R Konze
Nov 1, 1971·Analytical Biochemistry·C Beauchamp, I Fridovich
Mar 1, 1964·Analytical Biochemistry·P M NAIR, C S VAIDYANATHAN

Citations

Jan 1, 1978·Progress in Biophysics and Molecular Biology·B Halliwell
Apr 23, 2002·The Plant Journal : for Cell and Molecular Biology·Rochus FrankeClint Chapple
Jan 1, 1977·Plant Physiology·H A Stafford, M A Brown
Oct 1, 1978·Photochemistry and Photobiology·I Fridovich
Dec 30, 2008·Free Radical Biology & Medicine·B Halliwell
Apr 16, 2015·Journal of Experimental Botany·Tinne BoeckxAlison H Kingston-Smith
Jan 1, 1987·CRC Critical Reviews in Biochemistry·J V BannisterG Rotilio
Mar 21, 2017·Frontiers in Plant Science·Tinne BoeckxAlison H Kingston-Smith

Related Concepts

Magnorbin
Caffeic Acids
Catalase T
Chlorophyll
Etioplasts
Cinnamates
Coumaric Acids
Free Radicals
Light
NADH

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