Hydroxynonenal inactivates cathepsin B by forming Michael adducts with active site residues

Protein Science : a Publication of the Protein Society
John W CrabbH F Hoff

Abstract

Oxidation of plasma low-density lipoprotein (oxLDL) generates the lipid peroxidation product 4-hydroxy-2 nonenal (HNE) and also reduces proteolytic degradation of oxLDL and other proteins internalized by mouse peritoneal macrophages in culture. This leads to accumulation of undegraded material in lysosomes and formation of ceroid, a component of foam cells in atherosclerotic lesions. To explore the possibility that HNE contributes directly to the inactivation of proteases, structure-function studies of the lysosomal protease cathepsin B have been pursued. We found that treatment of mouse macrophages with HNE reduces degradation of internalized maleyl bovine serine albumin and cathepsin B activity. Purified bovine cathepsin B treated briefly with 15 microM HNE lost approximately 76% of its protease activity and also developed immunoreactivity with antibodies to HNE adducts in Western blot analysis. After stabilization of the potential Michael adducts by sodium borohydride reduction, modified amino acids were localized within the bovine cathepsin B protein structure by mass spectrometric analysis of tryptic peptides. Michael adducts were identified by tandem mass spectrometry at cathepsin B active site residues Cys 29 (mature A c...Continue Reading

References

Jun 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·K Uchida, E R Stadtman
Jun 22, 1992·Biochimica Et Biophysica Acta·W JessupR T Dean
Dec 1, 1991·The Journal of Clinical Investigation·J L Witztum, D Steinberg
Jan 1, 1991·Free Radical Biology & Medicine·H EsterbauerH Zollner
May 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·M E Haberland, A M Fogelman
Sep 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·K UchidaE R Stadtman
Jan 1, 1996·Free Radical Biology & Medicine·D Yin
Nov 8, 1996·The Journal of Biological Chemistry·M S BolgarS J Gaskell
Jan 10, 1997·The Journal of Biological Chemistry·C IllyJ S Mort
Apr 1, 1997·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·D W DavisA Holian
Apr 25, 1997·Biochemical Pharmacology·D L Vander JagtR E Royer
Mar 21, 1998·Archives of Biochemistry and Biophysics·A Del CorsoU Mura
Apr 16, 1998·Protein Science : a Publication of the Protein Society·J W CrabbD Bok

❮ Previous
Next ❯

Citations

Sep 25, 2004·Mass Spectrometry Reviews·Isabella Dalle-DonneAldo Milzani
Jan 6, 2005·Springer Seminars in Immunopathology·Sudhir PaulMarc E Weksler
Jul 11, 2006·Parasitology Research·Thomas D Lockwood
Oct 22, 2010·Amino Acids·Ram H NagarajAlan W Stitt
Feb 26, 2004·Free Radical Biology & Medicine·Golnar Kamalvand, Zafer Ali-Khan
Apr 23, 2003·Biochemical and Biophysical Research Communications·Kaori NishikawaKeiji Wada
Aug 2, 2003·Molecular Aspects of Medicine·Anne Negre-SalvayreRobert Salvayre
Nov 17, 2011·Chemical Research in Toxicology·Christopher J MartyniukRichard M Lopachin
May 10, 2006·The Journal of Biological Chemistry·Carlos BatthyanyBruce A Freeman
Aug 5, 2006·The Journal of Biological Chemistry·Taixing CuiYuqing E Chen
Feb 21, 2008·The Journal of Biological Chemistry·Bruce A FreemanMarco d'Ischia
Apr 26, 2008·Molecular & Cellular Proteomics : MCP·Kwok-Peng NgJohn W Crabb
Aug 28, 2004·Molecular Biology of the Cell·Roberta KiffinAna Maria Cuervo
Dec 18, 2007·Toxicological Sciences : an Official Journal of the Society of Toxicology·Richard M LoPachinTerrence Gavin
Sep 26, 2009·Toxicology Mechanisms and Methods·R Patrick RaineyAnthony P Albino
Apr 23, 2013·Journal of Photochemistry and Photobiology. B, Biology·Sarah D Lamore, Georg T Wondrak
May 5, 2009·Biochimica Et Biophysica Acta·Josephine V Glenn, Alan W Stitt
May 31, 2015·Neurobiology of Disease·Catherine A Cobb, Marsha P Cole
Dec 18, 2007·Biochemical and Biophysical Research Communications·Wataru AoiToshikazu Yoshikawa
Nov 28, 2006·Chemistry and Physics of Lipids·Viral V BrahmbhattDavid A Ford
Jun 13, 2012·Journal of Mass Spectrometry : JMS·Qingyuan LiuScott Gronert
Aug 12, 2004·Free Radical Biology & Medicine·Martin D BrandNadeene Parker
Jun 27, 2006·Experimental Eye Research·Tia EsteyVasilis Vasiliou
Nov 18, 2005·Immunology Letters·Sudhir PaulHiroaki Taguchi
Dec 9, 2004·The Journal of Veterinary Medical Science·Jin-Wook JungSeung Yong Hwang
Jul 16, 2014·Free Radical Biology & Medicine·Oleksii A SkorokhodEvelin Schwarzer
Feb 10, 2009·Free Radical Biology & Medicine·Paul R S BakerBruce A Freeman
Feb 17, 2017·The FEBS Journal·Tommy Weiss-SadanGalia Blum
Feb 20, 2007·Proteomics·Surendranath P SumanDaniel C Liebler
May 11, 2004·Mass Spectrometry Reviews·Marina CariniRoberto Maffei Facino
Feb 18, 2003·Arteriosclerosis, Thrombosis, and Vascular Biology·Henry F HoffRobert L Salomon

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.