Hydroxypyruvate reductase with a carboxy-terminal targeting signal to microbodies is expressed in Arabidopsis

Plant & Cell Physiology
S ManoM Nishimura

Abstract

Five Arabidopsis EST cDNA clones of hydroxypyruvate reductase (HPR), a photorespiratory enzyme in leaf peroxisomes, were sequenced. Deduced amino acid sequences revealed that HPR in Arabidopsis contained the carboxy-terminal targeting signal to microbodies. Nucleotide sequence analysis showed that the cDNA with the longest insert contained an open reading frame of 1,158 bp which encoded a polypeptide with 386 amino acids with a calculated molecular mass of 42,251 Da. A Southern blot analysis suggested that the Arabidopsis HPR gene, like that of the pumpkin HPR gene, exists as a single copy. Two kinds of pumpkin HPR mRNA might be produced from a single gene by alternative splicing, but the structure of the genomic DNA indicated that the Arabidopsis HPR gene did not undergo alternative splicing. We detected a polypeptide with a molecular mass of 42 kDa in green leaves of Arabidopsis using an HPR-specific antibody. Immunoelectron microscopy revealed that Arabidopsis HPR protein was exclusively localized in leaf peroxisomes in green leaves. These results indicate that HPR is expressed in a form with a carboxy-terminal targeting signal to microbodies and is localized in microbodies in Arabidopsis, suggesting that the differences in ...Continue Reading

Citations

Aug 29, 2003·The Plant Journal : for Cell and Molecular Biology·Akane KamigakiMuneharu Esaka
Feb 14, 2009·Protoplasma·Akiko ShinozakiYasuko Hayashi
Mar 13, 2003·The Plant Journal : for Cell and Molecular Biology·Daisuke IgarashiChieko Ohsumi
Jul 4, 2006·The Plant Journal : for Cell and Molecular Biology·Shoji ManoMikio Nishimura
Jan 10, 2014·Journal of Integrative Plant Biology·Nenghui YeXinxiang Peng
Jul 19, 2013·Biochimica Et Biophysica Acta·Francisco J CorpasJuan B Barroso
Feb 24, 2006·Vitamins and Hormones·Shoji Mano, Mikio Nishimura
Mar 31, 1999·The Plant Journal : for Cell and Molecular Biology·S ManoM Nishimura
Feb 19, 2008·Plant & Cell Physiology·Yuko AraiMikio Nishimura
Jan 28, 2014·Journal of Cell Science·Kohki YoshimotoYoshinori Ohsumi

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