Hydroxysafflor Yellow A Attenuates Hydrogen Peroxide-Induced Oxidative Damage on Human Umbilical Vein Endothelial Cells

Evidence-based Complementary and Alternative Medicine : ECAM
Yuefeng XieBo Jin

Abstract

Oxidative stress of endothelial cells is thought to be a principal cause that induces many cardiovascular diseases. Hydroxysafflor yellow A (HSYA) is a major active component in traditional Chinese medicine safflower and has been used to cure ischemic cardiovascular diseases in China for many years. This study aims to investigate whether HSYA has a repairing effect on oxidative damage of human umbilical vein endothelial cells (HUVECs) induced by H2O2 and to provide a theoretical basis for the clinical treatment of cardiovascular diseases related to traditional Chinese medicine. Based on the establishment of an H2O2-induced HUVEC oxidative injury model, the cell viability and proliferation rate were measured by the MTT assay and EdU staining. The intracellular GSH/GSSG ratio and SOD activity were determined by kits. The ROS level was detected by flow cytometry. And the BAX, Bcl-2, PTEN, and AKT expressions were evaluated with western blotting methods. The results showed that HSYA treatment significantly attenuated the H2O2-induced HUVEC cell damage, increased the intracellular GSH/GSSG ratio and unit SOD activity also, and decreased the intracellular ROS levels. Furthermore, HSYA increased the expressions of AKT and Bcl-2 protei...Continue Reading

References

Jan 1, 1996·Journal of Atherosclerosis and Thrombosis·A Karsan, J M Harlan
Mar 5, 1999·Science·D C Wallace
Apr 14, 1999·Proceedings of the National Academy of Sciences of the United States of America·L C Cantley, B G Neel
Mar 22, 2000·Circulation Research·S Dimmeler, A M Zeiher
Feb 13, 2001·Biochemical and Biophysical Research Communications·T IantomasiM T Vincenzini
Mar 12, 2002·Methods in Enzymology·Dean P Jones
Mar 21, 2003·The American Journal of Cardiology·David HarrisonHelmut Drexler
Jun 24, 2003·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Danyelle M TownsendHaim Tapiero
Dec 4, 2003·Biochemical Society Transactions·H NohlK Staniek
Oct 12, 2004·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Jian-Mei Li, Ajay M Shah
Jan 15, 2005·Antioxidants & Redox Signaling·Irfan RahmanH J Forman
Sep 2, 2008·Molecular Aspects of Medicine·Saibal K Biswas, Irfan Rahman
Feb 4, 2009·Antioxidants & Redox Signaling·Konstantin G Birukov
Jul 12, 2011·Inflammatory Bowel Diseases·Serena CatarziTeresa Iantomasi
Sep 22, 2012·Biochimica Et Biophysica Acta·Shelly C Lu
Jun 5, 2014·Current Molecular Medicine·D Vara, G Pula
Jan 20, 2016·Oxidative Medicine and Cellular Longevity·Claudio Cabello-VerrugioFelipe Simon
Oct 25, 2016·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Jens ZierleFlorian Lang
May 11, 2017·Molecular Medicine Reports·Ronghui HanHongxin Wang
May 23, 2017·Cancer Cell·Yiqun ZhangChad J Creighton
Sep 19, 2017·Current Atherosclerosis Reports·Ajoe John KattoorJawahar L Mehta
Sep 7, 2019·Nutrients·Thomas Senoner, Wolfgang Dichtl

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Citations

Aug 18, 2021·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·Xinyan XueYunxia Li

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Methods Mentioned

BETA
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flow cytometry

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