Hydroxysafflor yellow a protects brain microvascular endothelial cells against oxygen glucose deprivation/reoxygenation injury: Involvement of inhibiting autophagy via class I PI3K/Akt/mTOR signaling pathway

Brain Research Bulletin
Guang YangHuangzheng Liang

Abstract

The present study aimed to test whether Hydroxysafflor yellow A (HSYA) protects the brain microvascular endothelial cells (BMECs) injury induced by oxygen glucose deprivation/reoxygenation (OGD/R) via the PI3K/Akt/mTOR autophagy signaling pathway. Primary rat BMECs were cultured and identified by the expression of factor VIII-related antigen before being exposed to OGD/R to imitate ischemia/reperfusion (I/R) damage in vitro. The protective effect of HSYA was evaluated by assessing (1) cellular morphologic and ultrastructural changes; (2) cell viability and cytotoxicity; (3) transendothelial electrical resistance (TEER) of monolayer BMECs; (4) cell apoptosis; (5) fluorescence intensity of LC3B; (6) LC3 mRNA expression; (7) protein expressions of LC3, Beclin-1, Zonula occludens-1 (ZO-1), phospho-Akt (p-Akt), Akt, phospho-mTOR (p-mTOR) and mTOR. It was found that HSYA (20, 40, and 80 μM) and 3-MA effectively reversed the cellular morphological and ultrastructural changes, increased cell survival, normalized the permeability of BMECs, and suppressed apoptosis induced by OGD/R (2 h OGD followed by 24 h reoxygenation). Concurrently, HSYA and 3-MA also inhibited OGD/R-induced autophagy evidenced by the decreased number of autophagosom...Continue Reading

References

Nov 1, 1973·The Journal of Clinical Investigation·L W HoyerJ R Hoyer
Feb 24, 2000·The Journal of Pathology·D L Carden, D N Granger
Sep 19, 2000·Bioscience, Biotechnology, and Biochemistry·K KazumaT Okuno
Jan 30, 2004·Proceedings of the National Academy of Sciences of the United States of America·Charles R KeeseIvar Giaever
Jun 1, 2004·Journal of Cell Science·Yukiko KabeyaTamotsu Yoshimori
Apr 20, 2006·Journal of the National Cancer Institute·Shin-ichi YaguchiTakao Yamori
Dec 27, 2007·Acta Pharmacologica Sinica·Ya-ping YangZheng-hong Qin
Sep 16, 2008·Neurobiology of Disease·Karin E Sandoval, Ken A Witt
Feb 10, 2009·Methods in Enzymology·Päivi Ylä-AnttilaEeva-Liisa Eskelinen
Mar 28, 2009·Clinical Science·Wim MartinetGuido R Y De Meyer
Mar 31, 2009·International Journal of Molecular Sciences·Jia-Jun LiuRuo-Zhi Xiao
Jun 9, 2009·Brain Research·Katayun Cohen-Kashi MalinaVivian I Teichberg
Aug 6, 2010·Brain Research Reviews·Filipa Lourenço CardosoMaria Alexandra Brito
Sep 21, 2010·Neuroscience Letters·Melissa J SimonBarclay Morrison
Oct 12, 2010·Autophagy·FengFeng TianKoji Abe
Feb 12, 2011·Cell Death and Differentiation·R KangD Tang
Mar 28, 2012·CNS Neuroscience & Therapeutics·Ruoyang ShiJiming Kong
Jul 24, 2012·British Journal of Pharmacology·Bradlee L HeckmannJun Liu
Sep 13, 2012·Neuroscience Bulletin·Feng XuZheng-Hong Qin
Jan 11, 2013·Journal of Cellular and Molecular Medicine·Mayur V JainMarek Łos
Feb 15, 2013·The New England Journal of Medicine·Augustine M K ChoiBeth Levine
Sep 12, 2014·Neurochemical Research·Huizhen GuoXiaoping Lai
Jan 20, 2015·International Journal of Stroke : Official Journal of the International Stroke Society·Jilin Bai, Patrick D Lyden

❮ Previous
Next ❯

Citations

Jan 31, 2020·Neural Regeneration Research·Yun MoKang-Yong Liu
Nov 3, 2020·Frontiers in Cellular Neuroscience·Jie LiuYi Yang
Oct 30, 2020·Frontiers in Pharmacology·Xue BaiYu-Ping Tang
Mar 19, 2021·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·Ping HuangDennis Chang
Jul 9, 2021·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Wenzhe WangWenyuan Gao
Aug 15, 2021·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·Sajad FakhriJianbo Xiao
Aug 18, 2021·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·Xinyan XueYunxia Li

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Autophagosome

An autophagosome is the formation of double-membrane vesicles that involve numerous proteins and cytoplasmic components. These double-membrane vesicles are then terminated at the lysosome where they are degraded. Discover the latest research on autophagosomes here.

Autophagy & Model Organisms

Autophagy is a cellular process that allows degradation by the lysosome of cytoplasmic components such as proteins or organelles. Here is the latest research on autophagy & model organisms

Brain Injury & Trauma

brain injury after impact to the head is due to both immediate mechanical effects and delayed responses of neural tissues.

Autophagosome

An autophagosome is the formation of double-membrane vesicles that involve numerous proteins and cytoplasmic components. These double-membrane vesicles are then terminated at the lysosome where they are degraded. Discover the latest research on autophagosomes here.

AKT Pathway

This feed focuses on the AKT serine/threonine kinase, which is an important signaling pathway involved in processes such as glucose metabolism and cell survival.