Hydroxysteroid dehydrogenases in bovine and porcine granulosa cells convert zearalenone into its hydroxylated metabolites alpha-zearalenol and beta-zearalenol

Veterinary Research Communications
H MalekinejadJ Fink-Gremmels

Abstract

The enzymes 3alpha- and 3beta-hydroxysteroid dehydrogenase (3alpha- and 3beta-HSD) play a pivotal role in synthesis of various steroid hormones including oestradiol and testosterone. The structure of the mycotoxin zearalenone resembles many characteristics of steroids and binds to oestrogen receptors as an agonist. Consequently, it is suggested that zearalenone is also a substrate for 3alpha-HSD and 3beta-HSD. 3alpha-HSD and 3beta-HSD isoforms are expressed in the liver and kidney but also in many steroidogenic tissues. It was the aim of the present study to demonstrate the presence of these enzymes in granulosa cells, which were obtained from bovine and porcine ovaries, and to investigate whether zearalenone is a substrate for these enzymes. The results show a species-specific expression pattern in the granulosa cells of both species. Moreover, it was demonstrated that zearalenone when added to the culture medium, is converted into alpha-zearalenol and beta-zearalenol. Corresponding to the apparent expression profile, in porcine granulosa cells predominantly alpha-zearalenol was formed, whereas bovine granulosa cells preferentially converted zearalenone into beta-zearalenol. This is the first report demonstrating the extrahepa...Continue Reading

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Citations

Jul 14, 2011·Analytical and Bioanalytical Chemistry·Renata Gadzała-KopciuchBogusław Buszewski
Apr 3, 2010·Tropical Animal Health and Production·Malekinejad HassanBahrampour Kobra
Apr 16, 2013·Carbohydrate Research·Hannes MikulaJohannes Fröhlich
Mar 31, 2009·International Journal of Molecular Sciences·Fiorenza Minervini, Maria Elena Dell'Aquila
Oct 26, 2011·Toxicon : Official Journal of the International Society on Toxinology·Øyvind L BuskMorten Sørlie
Apr 29, 2016·Environmental Science and Pollution Research International·Intidhar Ben SalemSalwa Abid-Essefi
Sep 22, 2016·Tierärztliche Praxis. Ausgabe G, Grosstiere/Nutztiere·Théophile VissiennonEckard Schneider
Apr 21, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Marcin BryłaRenata Jędrzejczak
Jul 31, 2017·EFSA Journal·UNKNOWN EFSA Panel on Contaminants in the Food Chain (CONTAM)Isabelle P Oswald
Apr 21, 2021·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Fojan AgahiCristina Juan
May 20, 2020·Toxicon : Official Journal of the International Society on Toxinology·Magdalena Buszewska-Forajta

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