Hydroxytyrosol Ameliorates Endothelial Function under Inflammatory Conditions by Preventing Mitochondrial Dysfunction

Oxidative Medicine and Cellular Longevity
Nadia CalabrisoMaria Annunziata Carluccio

Abstract

Mitochondria are fundamental organelles producing energy and reactive oxygen species (ROS); their impaired functions play a key role in endothelial dysfunction. Hydroxytyrosol (HT), a well-known olive oil antioxidant, exerts health benefits against vascular diseases by improving endothelial function. However, the HT role in mitochondrial oxidative stress in endothelial dysfunction is not clear yet. To investigate the HT effects on mitochondrial ROS production in the inflamed endothelium, we used an in vitro model of endothelial dysfunction represented by cultured endothelial cells, challenged with phorbol myristate acetate (PMA), an inflammatory, prooxidant, and proangiogenic agent. We found that the pretreatment of endothelial cells with HT (1-30 μmol/L) suppressed inflammatory angiogenesis, a crucial aspect of endothelial dysfunction. The HT inhibitory effect is related to reduced mitochondrial superoxide production and lipid peroxidation and to increased superoxide dismutase activity. HT, in a concentration-dependent manner, improved endothelial mitochondrial function by reverting the PMA-induced reduction of mitochondrial membrane potential, ATP synthesis, and ATP5β expression. In PMA-challenged endothelial cells, HT also p...Continue Reading

References

Jan 1, 1990·Methods in Enzymology·H Esterbauer, K H Cheeseman
Feb 15, 2001·Biochemical Pharmacology·S LiekensJ Neyts
Aug 17, 2001·The Journal of Biological Chemistry·A Okado-Matsumoto, I Fridovich
Mar 5, 2003·Arteriosclerosis, Thrombosis, and Vascular Biology·Maria Annunziata CarluccioRaffaele De Caterina
Mar 9, 2004·Genes & Development·Daniel P Kelly, Richard C Scarpulla
Jun 17, 2004·Circulation·Jean Davignon, Peter Ganz
Apr 28, 2005·Free Radical Biology & Medicine·Scott W Ballinger
Nov 22, 2005·Journal of Biochemical and Biophysical Methods·Ana GomesJosé L F C Lima
Dec 6, 2005·Journal of Cellular Biochemistry·Richard C Scarpulla
Feb 7, 2006·Free Radical Biology & Medicine·María-Isabel CovasRafael de la Torre
Feb 17, 2006·The Journal of Nutritional Biochemistry·Javier S PeronaValentina Ruiz-Gutierrez
Apr 25, 2006·American Journal of Physiology. Heart and Circulatory Physiology·Michel Félétou, Paul M Vanhoutte
Apr 28, 2006·Journal of Agricultural and Food Chemistry·Mario Dell'AgliDonatella Caruso
Jan 24, 2007·American Journal of Physiology. Heart and Circulatory Physiology·David X Zhang, David D Gutterman
Apr 28, 2007·Circulation Research·Sean M Davidson, Michael R Duchen
Jun 26, 2007·American Journal of Physiology. Heart and Circulatory Physiology·Maria Annunziata CarluccioRaffaele De Caterina
Jun 10, 2008·Nature Protocols·Kristine M RobinsonJoseph S Beckman
May 12, 2009·American Journal of Physiology. Heart and Circulatory Physiology·Anna CsiszarZoltan Ungvari
May 22, 2010·Journal of Bioenergetics and Biomembranes·Valentina SenatoreGiuseppe Genchi
Oct 12, 2010·Biochimica Et Biophysica Acta·Richard C Scarpulla
May 20, 2011·Nature·Peter Carmeliet, Rakesh K Jain
Jul 23, 2011·Free Radical Biology & Medicine·Sergey Dikalov
Sep 14, 2011·Free Radical Biology & Medicine·Julia M SantosRenu A Kowluru
Sep 20, 2011·Cell·Michael PotentePeter Carmeliet
Oct 27, 2011·Free Radical Biology & Medicine·Balaraman KalyanaramanHarry Ischiropoulos
Nov 4, 2011·Trends in Endocrinology and Metabolism : TEM·Ian S Patten, Zolt Arany
Mar 7, 2012·Cardiovascular Research·Emma YuMartin Bennett
Nov 20, 2012·Annual Review of Physiology·Peter Dromparis, Evangelos D Michelakis
Jan 22, 2013·The International Journal of Biochemistry & Cell Biology·Lucie DulucRamaroson Andriantsitohaina
Mar 2, 2013·Molecular Nutrition & Food Research·Sandra Martín-PeláezIgor Pravst
Dec 5, 2013·Blood·Young-Woong Kim, Tatiana V Byzova
May 6, 2014·The International Journal of Biochemistry & Cell Biology·Lucie DulucGilles Simard
May 17, 2014·Frontiers in Physiology·Xiaoqiang TangDe-Pei Liu
May 23, 2014·Current Biology : CB·Michael Schieber, Navdeep S Chandel
May 29, 2014·Oxidative Medicine and Cellular Longevity·Shasha XingSi Jin
May 31, 2014·Archives of Biochemistry and Biophysics·Cristian Sandoval-AcuñaHernán Speisky
Jul 8, 2014·Oxidative Medicine and Cellular Longevity·Antonio AyalaSandro Argüelles

❮ Previous
Next ❯

Citations

May 30, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Ana Karković MarkovićCvijeta Jakobušić Brala
Jun 28, 2019·British Journal of Pharmacology·Lukas SchwingshacklGeorg Hoffmann
Mar 18, 2020·American Journal of Hypertension·Daniela MenichiniMonica Longo
Apr 23, 2020·Antioxidants·Sander BekeschusMatthias B Stope
Sep 16, 2018·International Journal of Molecular Sciences·Tanila Wood Dos SantosMarcelo Lima Ribeiro
Jan 10, 2020·Oxidative Medicine and Cellular Longevity·Giorgio AquilaPaola Rizzo
Aug 14, 2020·Nutrients·Jorge A Ramírez-TejeroEva Siles
May 27, 2020·Cardiovascular Therapeutics·Flavia FranconiAnnalisa Romani
Oct 8, 2020·Nutritional Neuroscience·Francesco VisioliEmma Burgos-Ramos
Aug 3, 2021·Oxidative Medicine and Cellular Longevity·Leila ChodariAziz Eftekhari
Aug 8, 2021·Foods·Lucia Melguizo-RodríguezOlga García-Martínez

❮ Previous
Next ❯

Methods Mentioned

BETA
PMA
fluorescence microscopy
MDA
PCR

Software Mentioned

CFX Manager
Optimas Image
Scion Image Alpha

Related Concepts

Related Feeds

Autophagy & Aging: Inhibitors

The feed focuses on the role of nuclear export inhibitors and their effect on autophagy and the aging process.