Hydroxytyrosol inhibits the inflammatory response of osteoarthritis chondrocytes via SIRT6-mediated autophagy.

Molecular Medicine Reports
Li-Qiang ZhiJian-Bing Ma

Abstract

Osteoarthritis (OA) is a common degenerative joint disease. Inflammation may exaggerate the catabolism and degeneration in the pathogenesis of OA. Hydroxytyrosol (HT) has been used in the management of inflammatory diseases. In addition, reports have revealed that autophagy was a therapeutic target of diseases caused by inflammation. Sirtuin 6 (SIRT6) has also been demonstrated to prevent OA development by reducing both the inflammatory response and chondrocyte senescence. However, the roles of SIRT6 and autophagy in cartilage and its underlying anti‑inflammatory mechanism are unknown. Therefore, the present study aimed to determine the effects of HT on autophagy and inflammation in chondrocytes, and clarify whether HT regulates the inflammatory response through SIRT6‑mediated autophagy. The expression of protein and mRNA were determined by western blot analysis and reverse transcription‑quantitative polymerase chain reaction. The production of cytokines was detected by ELISA. It was demonstrated that HT inhibited the levels of interleukin (IL)‑1β and IL‑6 in tumor necrosis factor (TNF)‑α‑stimulated chondrocytes in a concentration‑dependent manner. In addition, HT promoted cell autophagy and increased the mRNA and protein expre...Continue Reading

References

Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Aug 25, 2004·Osteoarthritis and Cartilage·Maria Cristina HonoratiAndrea Facchini
Aug 5, 2005·Molecular Biology of the Cell·Eriko MichishitaIzumi Horikawa
Apr 27, 2010·Nutrition Reviews·Sergio Granados-PrincipalM Carmen Ramirez-Tortosa
Dec 8, 2011·Arthritis and Rheumatism·Hiroshi SasakiRyosuke Kuroda
May 10, 2013·The New England Journal of Medicine·Augustine M K ChoiBeth Levine
May 10, 2013·The New England Journal of Medicine·Andrea DoriaLeonardo Punzi
Nov 12, 2013·Biochemical and Biophysical Research Communications·Jiangtao LinYanping Zheng
Dec 25, 2013·The Journal of Immunology : Official Journal of the American Association of Immunologists·Naoki TakasakaKazuyoshi Kuwano
Feb 25, 2015·Arthritis & Rheumatology·Beatriz CaramésMartin K Lotz
May 16, 2015·The International Journal of Biochemistry & Cell Biology·Mingcai ZhangJinxi Wang
Jul 30, 2015·Autophagy·Romana T Netea-MaierMihai G Netea
Oct 7, 2015·Nature Reviews. Rheumatology·Ewa M Roos, Nigel K Arden

❮ Previous
Next ❯

Citations

Feb 20, 2020·International Journal of Molecular Sciences·Manuela LeriMassimo Stefani
Sep 29, 2020·Frontiers in Medicine·Paula GratalAránzazu Mediero
Jul 11, 2018·Frontiers in Physiology·Claire VinatierBeatriz Caramés
Sep 18, 2020·Biology·Margot NeefjesPeter M van der Kraan

❮ Previous
Next ❯

Related Concepts

Related Feeds

Anti-inflammatory Treatments

A drug or substance that reduces inflammation (redness, swelling, and pain) in the body. Anti-inflammatory agents block certain substances in the body that cause inflammation and swelling. Discover the latest research on anti-inflammatory treatments here

Autophagy & Disease

Autophagy is an important cellular process for normal physiology and both elevated and decreased levels of autophagy are associated with disease. Here is the latest research.

Autophagy & Model Organisms

Autophagy is a cellular process that allows degradation by the lysosome of cytoplasmic components such as proteins or organelles. Here is the latest research on autophagy & model organisms