Hyperacidification of Citrus fruits by a vacuolar proton-pumping P-ATPase complex

Nature Communications
Pamela StrazzerFrancesca M Quattrocchio

Abstract

The sour taste of Citrus fruits is due to the extreme acidification of vacuoles in juice vesicle cells via a mechanism that remained elusive. Genetic analysis in petunia identified two vacuolar P-ATPases, PH1 and PH5, which determine flower color by hyperacidifying petal cell vacuoles. Here we show that Citrus homologs, CitPH1 and CitPH5, are expressed in sour lemon, orange, pummelo and rangpur lime fruits, while their expression is strongly reduced in sweet-tasting "acidless" varieties. Down-regulation of CitPH1 and CitPH5 is associated with mutations that disrupt expression of MYB, HLH and/or WRKY transcription factors homologous to those activating PH1 and PH5 in petunia. These findings address a long-standing enigma in cell biology and provide targets to engineer or select for taste in Citrus and other fruits.

References

Aug 30, 1994·Proceedings of the National Academy of Sciences of the United States of America·J M DaviesD Sanders
May 9, 1997·The Journal of Biological Chemistry·M L MüllerL Taiz
Dec 8, 1998·The Journal of Membrane Biology·A BruneE Echeverria
Apr 10, 1999·The Journal of Biological Chemistry·M L MüllerL Taiz
Feb 12, 2002·Nature Reviews. Molecular Cell Biology·Tsuyoshi Nishi, Michael Forgac
Mar 14, 2002·The Journal of Membrane Biology·M L Müller, L Taiz
May 11, 2005·Trends in Plant Science·Ronald KoesFrancesca Quattrocchio
Jan 28, 2006·Planta·Takehiko ShimadaEduardo Blumwald
Jun 1, 1989·Plant Physiology·E Echeverria, J K Burns
Apr 7, 2007·FEBS Letters·Roberto A GaxiolaKarin Schumacher
Nov 13, 2007·Journal of Food Science·Edith Ramos Ramos Da Conceicao NetaRoger F McFeeters
Apr 22, 2008·Nucleic Acids Research·A DereeperO Gascuel
May 10, 2008·Genome Génome / Conseil National De Recherches Canada·D Q FangM L Roose
Nov 11, 2008·Nature Cell Biology·Walter VerweijFrancesca Quattrocchio
Sep 2, 2009·Journal of Agricultural and Food Chemistry·Russell L RouseffFatima Jabalpurwala
May 11, 2011·Functional & Integrative Genomics·Alessio AprileMikeal L Roose
Jan 5, 2012·The Journal of Biological Chemistry·Florian RienmüllerRainer Hedrich
Nov 28, 2012·Nature Genetics·Qiang XuYijun Ruan
Sep 1, 1983·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·P de VlamingH Wiering
Jun 6, 2014·Nature Communications·Shahar CohenArthur A Schaffer
Oct 6, 2014·Current Opinion in Plant Biology·Karin Schumacher
Apr 22, 2015·The Plant Journal : for Cell and Molecular Biology·Ingo AppelhagenBernd Weisshaar
May 24, 2016·The New Phytologist·Yanbang LiFrancesca Quattrocchio
Jul 7, 2016·Developmental Biology·Margaret H Frank, Daniel H Chitwood
Jun 22, 2017·Cell Reports·Marianna FaracoFrancesca M Quattrocchio
Feb 8, 2018·Nature·Guohong Albert WuManuel Talon

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Citations

Oct 16, 2019·Frontiers in Plant Science·Avi SadkaEduardo Blumwald
Dec 11, 2019·International Journal of Molecular Sciences·Li-Jun LiHui Ni
Dec 6, 2019·Frontiers in Nutrition·Djin Gie Liem, Catherine Georgina Russell
Apr 6, 2019·Journal of Experimental Botany·Misha PaauwFrancesca M Quattrocchio
Aug 8, 2020·Journal of the Science of Food and Agriculture·Domenico CautelaNunzia DʼOnofrio
Jan 2, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Domenico CautelaDomenico Castaldo
Nov 1, 2020·Plant Molecular Biology·Lu WangYuepeng Han
Jan 15, 2021·Proceedings of the National Academy of Sciences of the United States of America·Lingcheng ZhuMingjun Li
Jan 30, 2021·Plant Communications·Muhammad Junaid RaoQiang Xu
Mar 30, 2021·The Plant Journal : for Cell and Molecular Biology·Pascal HolzheuUrsula Kummer
May 23, 2021·Molecular Plant·Liao LiaoYuepeng Han
Jul 22, 2021·AoB Plants·Kadeem J Gilbert, Tanya Renner
Jul 28, 2021·Nature Communications·Guohong Albert WuDaniel S Rokhsar

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Methods Mentioned

BETA
RNA-seq
PCR
electrophoresis
PCRs
confocal microscopy

Software Mentioned

GBLOCKS
MUSCLE
BLAST
Clustal
BOXSHADE
PHYML
Aliview

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