Hypercysteinemia promotes atherosclerosis by reducing protein S-nitrosylation

Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie
Yulong ChenEnqi Liu

Abstract

Protein S-nitrosylation plays important role in the regulation of cardiovascular functions in nitric oxide (NO) Pathway. Hypercysteinemia (HHcy) is an independently risk factor for atherosclerosis. We hypothesized that HHcy promotes atherosclerosis by reducing level of vascular protein S-nitrosylation. The aim of present study is to investigate effect of HHcy on vascular protein S-nitrosylation. A total of 45 male apoE-/- mice were randomly divided into three groups. The control group was fed a Western-type diet. The HHcy group was fed a diet containing 4.4% L-methionine, and the HHcy+NONOate group was fed a diet containing 4.4% L-methionine and administrated NONOate (ip). Human umbilical vein endothelial cells were performed for in vitro experiment. Plasma lipids were measured every 4 weeks. After 12 weeks, aortic atherosclerotic lesion areas were detected as well as cellular components. The levels of plasma homocysteine (Hcy) and NO were measured. S-nitrosylation was detected using immunofluorescence, and further confirmed by biotin switch method. We found that compared with the control group, Hcy levels, and atherosclerotic plaque, and content of vascular smooth muscle cells and macrophages in lesions significantly increased...Continue Reading

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Sep 6, 2014·International Journal of Molecular Medicine·Yulong ChenEnqi Liu

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Citations

Aug 31, 2017·Journal of the American College of Nutrition·Huizi TianWeidong Zhang
Sep 29, 2020·Neurochemical Research·Yadi ZhangYumiao Lang
Apr 19, 2018·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Guangwei ZhangRong Wang
Oct 30, 2021·Molecular Medicine Reports·Yali ZhangEnqi Liu

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