PMID: 7334130Jan 1, 1981Paper

Hypergammaglobulinemia and erythrocyte autoantibody complicate enzyme immunoassay of antimalarial antibody

Journal of Immunoassay
K W HunterW A Blackburn

Abstract

Enzyme immunoassay of specific antimalarial antibody in sera from Plasmodium yoelii-infected mice was complicated by hypergammaglobulinemia and erythrocyte (RBC) autoantibody. Malarious serum had higher immunoglobulin levels which resulted in a substantial nonspecific adsorption of antibody to antigen uncoated microliter wells even in the presence of a surfactant. Since it was possible that some of the antibody binding to solid phase-bound malarial antigen was also due to nonspecific adsorption, the antibody binding of nonimmune serum was compared to that of malarial immune serum diluted to an equivalent immunoglobulin concentration. This allowed differentiation of specific antimalarial antibody binding from nonspecific adsorption of immunoglobulin. Although malarial antigen was partially purified from intraerythrocytic parasites, it bound a significant amount of rabbit anti-mouse RBC antibody, indicating the presence of residual RBC antigens. Serum from immune mice, but not nonimmune mouse serum, also showed substantial binding to RBC stroma antigen, suggesting that a component of the observed antibody binding to malarial antigen was anti-RBC antibody. Hypergammaglobulinemia and the induction of RBC autoantibody may both be re...Continue Reading

References

Jun 1, 1977·Experimental Parasitology·L G Poels, C C van Niekerk
Mar 22, 1975·British Medical Journal·A VollerE Engvall
Oct 16, 1975·Nature·B M Greenwood, R M Vick
Sep 1, 1965·Immunochemistry·G ManciniJ F Heremans
Jun 24, 1971·FEBS Letters·B K. Van Weemen, A H.W.M. Schuurs
Mar 1, 1965·The American Journal of Tropical Medicine and Hygiene·D C ABELEC B EVANS

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antimalarial Agents (ASM)

Antimalarial agents, also known as antimalarials, are designed to prevent or cure malaria. Discover the latest research on antimalarial agents here.

Antimalarial Agents

Antimalarial agents, also known as antimalarials, are designed to prevent or cure malaria. Discover the latest research on antimalarial agents here.