Hyperglycaemia exerts deleterious effects on late endothelial progenitor cell secretion actions

Diabetes & Vascular Disease Research : Official Journal of the International Society of Diabetes and Vascular Disease
Jie ZhangMin Cheng

Abstract

Endothelial progenitor cells (EPCs) play a fundamental role in tissue regeneration and vascular repair both by differentiating into endothelial cells and by secretion of vasoactive substances that promote angiogenesis and maintain vascular homeostasis. It has previously been shown that hyperglycaemia impairs early and late EPC functions, such as differentiation, proliferation and adhesion. However, its role in the regulation of the production of vasoactive substances in EPCs, especially in late EPCs, is less well defined. We investigated the effects of hyperglycaemia on the production of vasodilator, fibrinolytic and angiogenic growth factors, and also on the activity of superoxide dismutase (SOD) in late EPCs. For this purpose, late EPCs were incubated with different concentrations of D-glucose (5-40 mmol/L) for 24 hr. Levels of nitric oxide (NO), tissue plasminogen activator (t-PA), plasminogen activator inhibitor-1 (PAI-1), prostaglandin I(2) (PGI(2)), vascular endothelial growth factor (VEGF) and the activity of SOD were measured by enzyme-linked immunosorbent assay (ELISA). Under high glucose stress conditions, late EPCs exhibited lower levels of NO, t-PA, PAI-1, PGI(2) and VEGF compared to control medium (5 mmol/L glucose...Continue Reading

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Citations

Dec 31, 2015·Pediatric Research·Juan GuiFrauke von Versen-Höynck
Dec 24, 2015·Oxidative Medicine and Cellular Longevity·Mark D RossGeraint Florida-James
Feb 1, 2017·Vascular Pharmacology·Rosalinda MadonnaRaffaele De Caterina
Jul 19, 2017·Diabetes & Vascular Disease Research : Official Journal of the International Society of Diabetes and Vascular Disease·Hongyan KangXiaoyan Deng
Apr 20, 2014·Arteriosclerosis, Thrombosis, and Vascular Biology·Kai-Hang Yiu, Hung-Fat Tse

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Methods Mentioned

BETA
density gradient centrifugation
Fluorescence
enzyme-linked immunosorbent assay
ELISA
FACS

Software Mentioned

SPSS

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