Hyperglycemia Affects the Expression of Inflammatory Genes in Peripheral Blood Mononuclear Cells of Patients with Type 2 Diabetes

Immunological Investigations
Mohamad Akbari, Vahideh Hassan-Zadeh

Abstract

Innate immune system dysregulation and chronic low-grade inflammation are associated with the pathogenesis of type 2 diabetes (T2D). The aim of this study was to investigate the effect of hyperglycemia on mRNA expression of four inflammatory genes in peripheral blood mononuclear cells (PBMCs) of pre-diabetic and diabetic individuals. In a case-control study, quantitative real-time PCR was used to analyze changes in IL-1β, IL1R1, IL-6, and IL6ST gene expression in PBMCs of 30 T2D patients with high blood glucose levels, 24 diabetic and nondiabetic individuals with moderately high blood glucose levels and 30 controls with normal blood glucose levels. In T2D patients with high blood glucose, IL-1β expression showed a 2.69-fold increase (p = 0.0380), while IL-6 expression levels were 3.45 fold lower (p = 0.0045) versus control subjects. Moreover, compared with control group the expression of IL1R1 and IL-6 genes both were downregulated in individuals with moderately high blood glucose levels by 2.38 (p = 0.0365) and 4.34 fold (p = 0.0027), respectively. In addition, hemoglobin A1C (A1C) levels were positively correlated with IL-1β expression and fasting plasma glucose (FPG) levels showed a positive correlation with IL-1β and a nega...Continue Reading

References

May 1, 1987·The Journal of Experimental Medicine·S J WarnerP Libby
Mar 18, 1996·Biochemical and Biophysical Research Communications·J M StouthardH P Sauerwein
Jun 1, 1996·Hormone and Metabolic Research = Hormon- Und Stoffwechselforschung = Hormones Et Métabolisme·D ReinholdE D Schleicher
Apr 20, 2002·European Journal of Clinical Nutrition·N MitoK Sato
Nov 28, 2002·Diabetes·Joseph J SennRobert A Mooney
Jul 15, 2003·American Journal of Physiology. Endocrinology and Metabolism·Adam SteensbergBente Klarlund Pedersen
Oct 28, 2003·Diabetes·Peter J KloverRobert A Mooney
Nov 1, 2003·Biochemical and Biophysical Research Communications·Claire LagathuMartine Caron
Feb 3, 2005·Nature Medicine·Melek C ArkanMichael Karin
Apr 13, 2005·Lancet·Michael StumvollTimon W van Haeften
Apr 12, 2007·American Journal of Physiology. Endocrinology and Metabolism·Mohan R DasuIshwarlal Jialal
Jun 21, 2007·Diabetes·Jan A EhsesMarc Y Donath
Jun 28, 2007·The Journal of Clinical Endocrinology and Metabolism·Ellen C KaizerPerrin C White
Jun 13, 2008·Nature Protocols·Thomas D Schmittgen, Kenneth J Livak
Oct 17, 2008·Physiological Reviews·Bente K Pedersen, Mark A Febbraio
Jan 7, 2009·Mediators of Inflammation·Panayoula C TsiotraSotirios A Raptis
Jan 15, 2011·Nature Reviews. Immunology·Marc Y Donath, Steven E Shoelson
May 7, 2014·Diabetes Research and Clinical Practice·Nathalie EsserNicolas Paquot
Apr 22, 2015·Nature Immunology·Christopher A Hunter, Simon A Jones
Apr 5, 2016·Immunity, Inflammation and Disease·Matthew P SpindlerSrinath Sanda
Mar 7, 2018·Inflammopharmacology·Mohamad Akbari, Vahideh Hassan-Zadeh

❮ Previous
Next ❯

Citations

Apr 10, 2019·Immunological Investigations·Humera InayatAkhter Ali Baloch
Nov 11, 2019·Inflammopharmacology·Mohamad Akbari, Vahideh Hassan-Zadeh
Jan 12, 2021·Saudi Journal of Biological Sciences·Noura M DarwishFatmah S AlQahtany
Jul 11, 2021·Diabetes Research and Clinical Practice·Shani TsameretOren Froy

❮ Previous
Next ❯

Related Concepts

Related Feeds

Biomarkers for Type 2 Diabetes

Biomarkers can help understand chronic diseases and assist in risk prediction for prevention and early detection of diseases. Here is the latest research on biomarkers in type 2 diabetes, a disease in which the body is unable to produce or properly use insulin.