Hyperglycemia and advanced glycosylation end products suppress adipocyte apoE expression: implications for adipocyte triglyceride metabolism.

American Journal of Physiology. Endocrinology and Metabolism
Doris Joy EspirituTheodore Mazzone

Abstract

Endogenous adipocyte apolipoprotein E (apoE) plays an important role in adipocyte lipoprotein metabolism and lipid flux. A potential role for hyperglycemia in regulating adipocyte apoE expression and triglyceride metabolism was examined. Exposure of adipocytes to high glucose or advanced glycosylation end product-BSA significantly suppressed apoE mRNA and protein levels. This suppression was significantly attenuated by antioxidants or inhibitors of the NF-κB transcription pathway. Hyperglycemia in vivo led to adipose tissue oxidant stress and significant reduction in adipose tissue and adipocyte apoE mRNA level. Incubation with antioxidant in organ culture completely reversed this suppression. Hyperglycemia also reduced adipocyte triglyceride synthesis, and this could be completely reversed by adenoviral-mediated increases in apoE. To more specifically evaluate an in vivo role for adipocyte apoE expression on organismal triglyceride distribution in vivo, WT or apoE knockout (EKO) adipose tissue was transplanted in EKO recipient mice. After 12 wk, WT adipocytes transplanted in EKO mice accumulated more triglyceride compared with transplanted EKO adipocytes. In addition, EKO recipients of WT adipose tissue had reduced hepatic tri...Continue Reading

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Feb 19, 2009·American Journal of Physiology. Endocrinology and Metabolism·Zhi Hua HuangTheodore Mazzone
May 30, 2009·Journal of Leukocyte Biology·Ravichandran RamasamyAnn Marie Schmidt

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Citations

May 20, 2014·Biological Reviews of the Cambridge Philosophical Society·Chen-Lu WuBi-Lian Yu
Dec 17, 2015·Foot & Ankle International·Victor A CheuyMichael J Mueller
Dec 26, 2019·Scientific Reports·Clarissa Strieder-BarbozaRobert W O'Rourke

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