Hyperglycemia and hypoxia are interrelated in their teratogenic mechanism: studies on cultured rat embryos

Birth Defects Research. Part B, Developmental and Reproductive Toxicology
Asher OrnoyNoa Bischitz

Abstract

Hyperglycemia and hypoxia are well-known teratogens that may affect many animal species, including man. One of the main mechanisms of teratogenic action of both seems to be increased oxidative stress. The purpose of this study was to evaluate the hypothesis that in the developing embryo hyperglycemia also leads to hypoxia, both resulting in oxidative damage. The study was performed on 10.5-day-old rat embryos of the regular Sabra strain cultured for 28 hours in hyperglycemic or hypoxic conditions. Embryonic growth and rate of anomalies was assessed at the end of the culture period. The embryonic oxidative damage was investigated by studying the levels of Malondialdehyde (MDA) to determine the lipid peroxidation. The redox status was studied by measuring the activity of Catalase-like (CAT) and Super Oxide Dismutase (SOD) enzymes and the amount of Low Molecular Weight Antioxidants (LMWA). In addition, we studied by immunohistochemistry in the embryos and yolk sacs the amount of nitrotyrosine as an additional marker for the extent of oxidative stress. The amounts of the redox and hypoxia sensitive transcription factors HIF1alpha, NFkB, and IkB were also studied by immunohistochemistry. Both hyperglycemia and hypoxia increased the ...Continue Reading

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Citations

Oct 12, 2012·In Vitro Cellular & Developmental Biology. Animal·Gladys Chirino-GalindoMartín Palomar-Morales
Aug 19, 2011·Orphanet Journal of Rare Diseases·Benjamin D Solomon
Oct 20, 2012·Toxicology and Applied Pharmacology·Zivanit ErgazAsher Ornoy
Jan 11, 2016·Birth Defects Research. Part B, Developmental and Reproductive Toxicology·Zivanit ErgazAsher Ornoy
Feb 3, 2012·Clinical Neurology and Neurosurgery·Mahshid Sadat Hosseini-ZareShahram Akrami
Aug 31, 2010·Birth Defects Research. Part B, Developmental and Reproductive Toxicology·Robert G Ellis-Hutchings, Edward W Carney
Jun 28, 2011·Birth Defects Research. Part B, Developmental and Reproductive Toxicology·Asher OrnoyRichard K Miller
Mar 19, 2015·Birth Defects Research. Part C, Embryo Today : Reviews·Asher OrnoyRichard Kermit Miller
Aug 31, 2018·Frontiers in Endocrinology·Radka Cerychova, Gabriela Pavlinkova

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