Hypericin-mediated photodynamic therapy induces apoptosis in K562 human leukemia cells through JNK pathway modulation

Molecular Medicine Reports
Yixiao XuKunyuan Guo

Abstract

Hypericin (Hyp) is traditionally used as an antidepressant and antiviral agent. It selectively accumulates in spheroids and is also used as a photosensitizer in the photodynamic therapy of cancer. The present study aimed to investigate the cytotoxic effect of Hyp‑mediated photodynamic therapy (Hyp‑PDT) on cell growth and apoptosis of K562 leukemia cells, and to examine the underlying mechanisms. Hyp‑PDT was performed with different light intensities (0.1, 0.3 and 0.5 mW/cm2), different concentrations of Hyp (0, 0.2, 0.4 and 0.8 µg/ml) and different durations of irradiation (0, 2, 4 and 8 min) in order to select the optimal conditions for subsequent experiments. A concentration of 0.4 µg/ml Hyp with a 5 h drug‑light interval and 4 min irradiation at 0.3 mW/cm2 light intensity was selected as the optimal conditions. The effects of Hyp‑PDT on apoptosis were determined by detecting morphological changes under microscopy and by performing western blot analysis. The results revealed that Hyp‑PDT suppressed cell viability in a light intensity‑, dose‑ and irradiation duration‑dependent manner. The expression levels of cleaved caspase‑9, cleaved caspase‑3 and phosphorylated‑C‑Jun N terminal kinase (JNK)l were significantly upregulated f...Continue Reading

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Citations

Apr 29, 2020·Photochemistry and Photobiology·David Kessel
Oct 23, 2019·Photodiagnosis and Photodynamic Therapy·M Ibrahim KhotDavid G Jayne
Apr 2, 2021·The Journal of Pharmacy and Pharmacology·Xiaoxv DongLuis J Cruz
Jan 20, 2021·ACS Biomaterials Science & Engineering·Mohammed Nadim SardoiwalaSubhasree Roy Choudhury

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Methods Mentioned

BETA
irradiating

Software Mentioned

SPSS
Openlab

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