Hypermethylated ERG as a cell-free fetal DNA biomarker for non-invasive prenatal testing of Down syndrome

Clinica Chimica Acta; International Journal of Clinical Chemistry
Xi ChenJinghui Ren

Abstract

Previous reports have shown that the ERG gene is hypermethylated in the placenta and hypomethylated in maternal blood cells. In this study, we explore the feasibility of hypermethylated ERG as a cell-free fetal (cff) DNA biomarker for non-invasive prenatal testing (NIPT) of Down syndrome. We randomly selected 90 healthy pregnant women, including 30 cases at each trimester of pregnancy. In addition, 15 pregnant women were recruited as the case group whose fetuses had been confirmed to have trisomy 21 by amniotic fluid analysis at 18th to 26th week gestation. Using HpaII, MspІ to digest cell-free maternal plasma DNA, we performed SYBR Green PCR to detect methylated sites of ERG sequences, and analyzed the concentrations of cff DNA in maternal plasma in different gestational trimesters and the case group. The ERG median concentrations of the maternal plasma after Hpa II digestion (LG copies/ml) in first, second and third-trimesters were 5.38, 6.10, and 7.04, respectively (Kruskal-Wallis, P<0.01); and that in the trisomy 21 case group was 6.85, which was higher than the second-trimester (Mann-Whitney, P<0.01). The study demonstrated that ERG gene is hypermethylated in cff DNA but hypomethylated in maternal DNA; and the median conce...Continue Reading

References

Aug 16, 1997·Lancet·Y M LoJ S Wainscoat
Sep 2, 1999·Biochemical and Biophysical Research Communications·I PogribnyS J James
Nov 24, 1999·Molecular Genetics and Metabolism·M HiratsukaM Mizugaki
Nov 18, 2000·Journal of the National Cancer Institute·K KawakamiS J Meltzer
Nov 20, 2002·American Journal of Obstetrics and Gynecology·Thomas LeeDiana W Bianchi
Aug 19, 2007·Reproductive Biomedicine Online·Robert W OldMaj Anita Hultén
Aug 12, 2009·Proceedings of the National Academy of Sciences of the United States of America·Elizabeth A KruseBenjamin T Kile
Jan 16, 2010·Biology of Reproduction·Maria Luz BellidoWolfgang Holzgreve
Feb 8, 2011·PloS One·Shimul ChowdhuryCharlotte A Hobbs
Feb 14, 2012·BMC Cancer·Naoyuki HanadaYasuo Saijo

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Citations

Aug 25, 2019·Cells·Marzena CiechomskaWlodzimierz Maslinski
Feb 25, 2020·BioMed Research International·Xiaofang CuiWeiyang Li

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