Hypermethylated LATS2 gene with decreased expression in female breast cancer: A case control study from North India

Gene
SadafSyed Akhtar Husain

Abstract

LATS2, a presumed tumor suppressor gene located on chromosome 13q11-12 is involved in cell growth related activity like regulation of cell cycle at G1/S. The reduced expression of LATS2 has been reported in many tumors; including tumors of Breast, which is to the best of our knowledge has not been studied in north Indian female breast cancer population. Here, we looked upon the expression pattern and methylation status of the LATS2 gene in north Indian female breast cancer cases to further strengthen its role as a tumor suppressor gene and more importantly as a cancer biomarker. mRNA expression level was determined by real time PCR in 140 Breast cancer patients, Protein expression was studied by Immunohistochemistry and Promoter methylation was studied by Methylation specific PCR. All findings were correlated with clinicopathological features. LATS2 mRNA expression was remarkably downregulated in 67.85% (95/140) cases. The expression of Large Associated Tumor Suppressor 2 at protein level was also absent in 67.85% (95/140) cases. The absence of LATS2 protein strongly correlated with promoter hypermethylation where 91 out of a total of 107 hyper methylated cases showed absence of protein (91/107, 85%). The absence of LATS2 prote...Continue Reading

References

Mar 8, 2003·The Journal of Biological Chemistry·Yasuhiko KamikuboToshiyuki Hori
Jul 11, 2003·Oncogene·Yunfang LiWufan Tao
Mar 5, 2005·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Yuri TakahashiShinzaburo Noguchi
Oct 24, 2007·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Lyndsay HarrisUNKNOWN American Society of Clinical Oncology
Oct 18, 2008·Circulation Research·Yutaka MatsuiJunichi Sadoshima
May 5, 2010·Genes & Development·Bin ZhaoKun-Liang Guan
Oct 19, 2010·Developmental Cell·Duojia Pan
Mar 1, 2010·Oncology Letters·Hidefumi SasakiYoshitaka Fujii
Jul 26, 2013·World Journal of Gastroenterology : WJG·Piotr M WierzbickiZbigniew Kmiec
Sep 16, 2014·International Journal of Cancer. Journal International Du Cancer·Jacques FerlayFreddie Bray
Jan 17, 2015·Nature Reviews. Cancer·Toshiro MoroishiKun-Liang Guan
Nov 22, 2015·Genes & Development·Noa FurthMoshe Oren
Jan 10, 2017·Nature·Adrian BritschgiMohamed Bentires-Alj
Aug 13, 2017·Medical Oncology·Carla Solange Escórcio-DouradoBenedito Borges da Silva

❮ Previous
Next ❯

Citations


❮ Previous
Next ❯

Related Concepts