Hypermethylated promoter region of DR3, the death receptor 3 gene, in rheumatoid arthritis synovial cells

Arthritis and Rheumatism
Nozomi TakamiShunichi Shiozawa

Abstract

To examine the promoter activity and protein expression of the death receptor 3 gene DR3, a member of the apoptosis-inducing Fas gene family, with particular reference to the methylation status of its promoter region in rheumatoid arthritis (RA). Genomic DNA was prepared from peripheral blood mononuclear cells obtained from healthy individuals and from patients with RA and synovial cells obtained from patients with RA and osteoarthritis. The methylation status of the DR3 promoter was analyzed by bisulfite genomic sequencing and methylation-specific polymerase chain reaction techniques. Gene promoter activity and protein expression were examined using the luciferase reporter and Western blotting techniques. The promoter region of the DR3 gene contained many CpG motifs, including one CpG island that was specifically hypermethylated in synovial cells from patients with RA. Promoter assays showed that the promoter CpG island was essential for the transactivation of the DR3 gene and that forced hypermethylation of the CpG island with the bacterial methylase Sss I in vitro resulted in inhibition of the DR3 gene expression. Furthermore, the expression of DR-3 protein was down-modulated in association with methylation of the promoter C...Continue Reading

References

Mar 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·M FrommerC L Paul
Apr 1, 1991·Molecular and Cellular Biology·J D SafferM B Annarella
Apr 1, 1995·Arthritis and Rheumatism·T NakajimaK Nishioka
Mar 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·M AdachiS Nagata
May 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·J H RussellR Wang
Sep 1, 1996·The Journal of Clinical Investigation·J WuJ D Mountz
Sep 3, 1996·Proceedings of the National Academy of Sciences of the United States of America·J G HermanS B Baylin
Nov 28, 1996·Nature·J KitsonS N Farrow
Nov 26, 1999·Gene·G Suske
Mar 22, 2001·The New England Journal of Medicine·E H Choy, G S Panayi
Dec 19, 2001·The Journal of Pathology·Manel Esteller, James G Herman
Jun 4, 2002·Nature Reviews. Genetics·Peter A Jones, Stephen B Baylin
Nov 9, 2002·Bioinformatics·Long-Cheng Li, Rajvir Dahiya
Apr 23, 2003·Current Opinion in Rheumatology·Anja BaierThomas Pap
Jul 9, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Kimihisa IchikawaTong Zhou
May 21, 2004·The New England Journal of Medicine·Nancy J Olsen, C Michael Stein
Nov 5, 2004·Cellular and Molecular Life Sciences : CMLS·A HermannA Jeltsch

❮ Previous
Next ❯

Citations

Aug 11, 2011·Inflammatory Bowel Diseases·Shunsuke SaitoKazuhide Yamamoto
Mar 23, 2011·Zeitschrift für Rheumatologie·A Jüngel, S Gay
Sep 28, 2013·Current Rheumatology Reports·Nunzio Bottini, Gary S Firestein
Aug 27, 2009·Clinical Reviews in Allergy & Immunology·Michelle TrenkmannSteffen Gay
Jan 19, 2011·Clinical Reviews in Allergy & Immunology·Marco FolciCarlo Selmi
Feb 1, 2011·Cellular & Molecular Immunology·Francesca MedaCarlo Selmi
May 5, 2009·Nature Reviews. Rheumatology·Emmanuel KarouzakisMichel Neidhart
Sep 10, 2010·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Gareth W JonesSimon A Jones
Oct 25, 2008·Arthritis Research & Therapy·Simon StrietholtSteffen Gay
Nov 21, 2012·Arthritis Research & Therapy·Kerstin KleinSteffen Gay
Oct 12, 2012·Biologics : Targets & Therapy·Judith M Greer, Pamela A McCombe
Dec 1, 2011·Epigenomics·Virginia C Rodriguez-CortezEsteban Ballestar
Mar 22, 2014·Cell Biochemistry and Biophysics·Feng-Lai YuanXiao-Gang Zhou
Mar 8, 2014·Expert Reviews in Molecular Medicine·Justine A EllisAnne-Louise Ponsonby
Jun 19, 2014·Molecular and Cellular Biochemistry·Anuradha KalaniNeetu Tyagi
Jan 14, 2016·Autoimmunity·Saeed AslaniMohammad Hossein Nicknam
Aug 1, 2012·Biochimie·Alexandre How KitJörg Tost
Dec 24, 2014·Immunology and Cell Biology·Ian A MacDonald, Nathaniel A Hathaway
Apr 27, 2012·Autoimmune Diseases·Paula Quintero-Ronderos, Gladis Montoya-Ortiz
Dec 23, 2016·Briefings in Bioinformatics·Mark T Mc AuleyJ Enrique Salcedo-Sora
Oct 11, 2016·Arthritis & Rheumatology·Brooke RheadLindsey A Criswell
Jan 14, 2017·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Majid AhmadiMehdi Yousefi
Nov 29, 2017·The Journal of Biological Chemistry·Akira HashiramotoShunichi Shiozawa
Oct 2, 2015·Therapeutic Advances in Musculoskeletal Disease·Adam CribbsUdo Oppermann
Jan 1, 2008·Arthritis and Rheumatism·R John LooneyLeonard H Calabrese
Dec 2, 2009·Arthritis and Rheumatism·Emmanuel KarouzakisMichel Neidhart
May 30, 2019·International Journal of Molecular Sciences·Marcin WłodarczykMarek Fol
Aug 25, 2019·Cells·Marzena CiechomskaWlodzimierz Maslinski
Apr 17, 2012·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Catherine BuiDavid A Young
Feb 26, 2016·Oncotarget·Aline C PlanelloDaniel D De Carvalho
Nov 28, 2012·Diabetes/metabolism Research and Reviews·Mary Ngoc DangPaolo Pozzilli
Nov 24, 2016·Clinical Reviews in Allergy & Immunology·Zhen WangZhiguang Zhou
Jul 21, 2017·Clinical Rheumatology·Patricia Castro-Santos, Roberto Díaz-Peña
Jul 6, 2018·Cellular and Molecular Life Sciences : CMLS·Zijun WangQianjin Lu
Oct 16, 2019·Frontiers in Immunology·Haijing WuQianjin Lu
May 13, 2020·Nature Reviews. Rheumatology·Gyrid Nygaard, Gary S Firestein

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis