Hypermethylation of calcitonin gene regulatory sequences in human breast cancer as revealed by genomic sequencing

International Journal of Cancer. Journal International Du Cancer
M HakkarainenJ Jänne

Abstract

DNA methylation has been studied intensively during the past years in order to elucidate its role in the regulation of gene expression, gene imprinting and cancer progression. Earlier studies have shown that a general genomic under-methylation is associated with chronic lymphocytic leukemia and metastatic prostate cancer. Site-specific methylation changes, as revealed by the use of methylation-sensitive restriction enzymes, have been reported to occur in the promotor region of the calcitonin gene in chronic myeloid leukemia as it progresses from the chronic phase to blast crisis, in non-Hodgkin's lymphoid neoplasms and in non-lymphocytic leukemia. We have now explored possible methylation changes associated with benign and malignant breast tumors. Two approaches were employed: (i) chemical determination of general genomic methylation status and (ii) base-specific analysis of the methylation changes in the promoter of the calcitonin gene with the aid of genomic sequencing. The results did not reveal any changes of total DNA 5-methylcytosine content in ductal carcinoma of breast in comparison with benign tumors. There was a small, yet significant, increase in 5-methylcytosine content in lobular carcinoma. Genomic sequencing of th...Continue Reading

References

Mar 11, 1992·Proceedings of the National Academy of Sciences of the United States of America·W E LohB E Weissman
Mar 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·M FrommerC L Paul
Apr 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·W S el-DeiryS B Baylin
Feb 28, 1983·Biochemical and Biophysical Research Communications·A P Feinberg, B Vogelstein
Jan 1, 1984·Human Genetics·J W HöppenerC J Lips
Jan 1, 1994·DNA Research : an International Journal for Rapid Publication of Reports on Genes and Genomes·Y NiwaY Machida
Jan 1, 1994·DNA Sequence : the Journal of DNA Sequencing and Mapping·S MyöhänenJ Jänne
Oct 11, 1994·Proceedings of the National Academy of Sciences of the United States of America·J G HermanW M Linehan
Sep 29, 1994·Nature·M BrandeisH Cedar

❮ Previous
Next ❯

Citations

May 17, 2000·Proceedings of the National Academy of Sciences of the United States of America·A T FergusonS Sukumar
May 18, 1999·Experimental Cell Research·M HabibA Niveleau
Nov 7, 2012·The Journal of Molecular Diagnostics : JMD·Rajasree ShanmuganathanKumaran Shanmugam
Aug 3, 2002·Oncogene·Melanie Ehrlich
Jun 11, 2011·International Journal of Immunopathology and Pharmacology·R MazzucchelliR Montironi
Feb 6, 2020·International Journal of Molecular Sciences·Sylwia PietrasikJanusz Blasiak

❮ Previous
Next ❯

Related Concepts

Related Feeds

B-Cell Leukemia (Keystone)

B-cell leukemia includes various types of lymphoid leukemia that affect B cells. Here is the latest research on B-cell leukemia.

Carcinoma, Ductal

Ductal carcinoma is a malignant neoplasm involving the ductal systems of any of a number of organs, such as the mammary glands, pancreas, prostate or lacrimal gland. Discover the latest research on ductal carcinoma here.

Cancer Genomics (Keystone)

Cancer genomics approaches employ high-throughput technologies to identify the complete catalog of somatic alterations that characterize the genome, transcriptome and epigenome of cohorts of tumor samples. Discover the latest research using such technologies in this feed.

Carcinoma, Lobular

Lobular carcinoma is an invasive type of breast cancer in which lobules, areas of the breast that produce milk, undergo malignant transformation. Here is the latest research.