Hypermethylation of MCAM gene is associated with advanced tumor stage in prostate cancer

The Prostate
Jun-Wei LiuDavid Sidransky

Abstract

DNA methylation has emerged as a promising biomarker for prostate cancer detection. In this report, we screened 36 candidate genes generated by a bioinformatic analysis of the human genome, and found that the melanoma cell adhesion molecule (MCAM) was an excellent candidate for cancer-specific methylation in prostate cancer. Direct sequencing of bisulfite-treated genomic DNA, conventional methylation-specific PCR (MSP), real-time quantitative methylation-specific PCR, immunohistochemistry, colony formation assay, and statistical analysis. We found that the melanoma cell adhesion molecule (MCAM) gene promoter was specifically methylated in prostate cancer cell lines and primary prostate cancer (PCa) but not in non-neoplastic prostate (BPH) tissues by direct sequencing of bisulfite-treated genomic DNA and conventional methylation-specific PCR (MSP). Further analysis with quantitative MSP showed greater hypermethylation of the MCAM promoter (80%, 70/88) in primary prostate cancer compared to 12.5% (3/24) in BPH. Prostatic intraepithelial neoplasias (PIN), potential precursors of prostate carcinoma, showed an intermediate methylation rate of 23% (7/30). We further observed that MCAM promoter methylation was directly correlated with...Continue Reading

References

Jun 1, 1992·The Journal of Pathology·K J DentonA L Harris
Dec 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·J M LehmannJ P Johnson
Sep 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·C SersJ P Johnson
Oct 9, 2001·Epidemiologic Reviews·H A Guess
Nov 22, 2001·Journal of the National Cancer Institute·C JerónimoD Sidransky
May 7, 2002·Nature Reviews. Cancer·David Sidransky
Apr 4, 2003·Lancet·Stephen FrankelDavid Neal
Jul 25, 2003·The New England Journal of Medicine·William G NelsonWilliam B Isaacs
Mar 18, 2004·Cancer Research·Srinivasan YegnasubramanianWilliam G Nelson
Dec 30, 2004·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Carmen JerónimoDavid Sidransky
Jan 20, 2005·Journal of the National Cancer Institute·Long-Cheng LiRajvir Dahiya
Feb 19, 2005·Nature Reviews. Cancer·Toshikazu Ushijima
Jan 24, 2007·CA: a Cancer Journal for Clinicians·Ahmedin JemalMichael J Thun
Jul 11, 2007·International Journal of Cancer. Journal International Du Cancer·Jun-Wei LiuDavid Sidransky

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Citations

Jun 26, 2013·Cancer Causes & Control : CCC·Chun ChaoMary Helen Black
Dec 26, 2012·Cancer Letters·Zhaoqing Wang, Xiyun Yan
Apr 7, 2010·Molecular and Cellular Biology·Howard DonningerGeoffrey J Clark
Jul 24, 2013·Molecular and Cellular Biology·Ping WangXiyun Yan
Feb 21, 2009·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Kimberly Laskie OstrowDavid Sidransky
Sep 23, 2014·International Journal of Molecular Sciences·Siri H StrandKarina D Sorensen
Apr 10, 2009·Biochimica Et Biophysica Acta·Allal OuhtitMadhwa G H Raj
Jul 30, 2014·Future Oncology·Luciane Tsukamoto KagoharaRafael Guerrero-Preston
Jul 30, 2011·Bioorganic & Medicinal Chemistry Letters·Sarah C HubbardCarolyn R Bertozzi
Apr 29, 2014·The Journal of Urology·Leonel MaldonadoDavid Sidransky
Jul 16, 2016·The Prostate·Kinga A KocembaPiotr Laidler
Aug 13, 2020·Signal Transduction and Targeted Therapy·Zhaoqing WangXiyun Yan
Nov 6, 2012·The International Journal of Biological Markers·Annamaria la TorreVito Michele Fazio

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