Hypermethylation of the DLC1 CpG island does not alter gene expression in canine lymphoma.

BMC Genetics
Jeffrey N BryanCharles W Caldwell

Abstract

This study is a comparative epigenetic evaluation of the methylation status of the DLC1 tumor suppressor gene in naturally-occurring canine lymphoma. Canine non-Hodgkin's lymphoma (NHL) has been proposed to be a relevant preclinical model that occurs spontaneously and may share causative factors with human NHL due to a shared home environment. The canine DLC1 mRNA sequence was derived from normal tissue. Using lymphoid samples from 21 dogs with NHL and 7 normal dogs, the methylation status of the promoter CpG island of the gene was defined for each sample using combined bisulfite restriction analysis (COBRA), methylation-specific PCR (MSP), and bisulfite sequencing methods. Relative gene expression was determined using real-time PCR. The mRNA sequence of canine DLC1 is highly similar to the human orthologue and contains all protein functional groups, with 97% or greater similarity in functional regions. Hypermethylation of the 5' and 3' flanking regions of the promoter was statistically significantly associated with the NHL phenotype, but was not associated with silencing of expression or differences in survival. The canine DLC1 is constructed highly similarly to the human gene, which has been shown to be an important tumor sup...Continue Reading

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Citations

Jul 14, 2012·PloS One·Mohammad Golam SabbirMichael R A Mowat
Apr 4, 2014·PloS One·Serena FerraressoLuca Aresu
Sep 7, 2014·Veterinary Immunology and Immunopathology·Masahiko SatoHajime Tsujimoto
Jun 8, 2012·Hematological Oncology·Laura MarconatoStefano Comazzi
May 13, 2014·Research in Veterinary Science·Aki Fujiwara-IgarashiHajime Tsujimoto
Apr 14, 2016·Stem Cells·Andrew M Hoffman, Steven W Dow
May 6, 2011·Diabetes Care·Jisun OhLawrence A Leiter
Mar 28, 2020·The Journal of Veterinary Medical Science·Hiroshi OhtaMitsuyoshi Takiguchi
Sep 15, 2020·Journal of Veterinary Internal Medicine·Cyril Parachini-WinterKaitlin M Curran
Oct 9, 2020·Journal of Leukocyte Biology·Chia-Hsin HsuChen-Si Lin
Nov 17, 2020·Frontiers in Oncology·Pedro Luiz Porfirio XavierHeidge Fukumasu

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Datasets Mentioned

BETA
FJ602870

Methods Mentioned

BETA
transfection
GTPase
PCR
biopsy

Software Mentioned

COBRA
SigScan
Methprimer
gel
MATLAB
BLAST
ClustalW

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