Hypermethylation of the hMLH1 gene promoter is associated with microsatellite instability in early human gastric neoplasia

Oncogene
A S FleisherS J Meltzer

Abstract

A significant portion of gastric cancers exhibit defective DNA mismatch repair, manifested as microsatellite instability (MSI). High-frequency MSI (MSI-H) is associated with hypermethylation of the human mut-L homologue 1 (hMLH1) mismatch repair gene promoter and diminished hMLH1 expression in advanced gastric cancers. However, the relationship between MSI and hMLH1 hypermethylation has not been studied in early gastric neoplasms. We therefore investigated hMLH1 hypermethylation, hMLH1 expression and MSI in a group of early gastric cancers and gastric adenomas. Sixty-four early gastric neoplasms were evaluated, comprising 28 adenomas, 18 mucosal carcinomas, and 18 carcinomas with superficial submucosal invasion but clear margins. MSI was evaluated using multiplex fluorescent PCR to amplify loci D2S123, D5S346, D17S250, BAT 25 and BAT 26. Methylation-specific PCR was performed to determine the methylation status of hMLH1. In two hypermethylated MSI-H cancers, hMLH1 protein expression was also evaluated by immunohistochemistry. Six of sixty-four early gastric lesions were MSI-H, comprising 1 adenoma, 4 mucosal carcinomas, and 1 carcinoma with superficial submucosal invasion. Two lesions (one adenoma and one mucosal carcinoma) dem...Continue Reading

References

May 1, 1992·Gut·M E CraanenG N Tytgat
Oct 17, 1991·The New England Journal of Medicine·J ParsonnetR K Sibley
May 7, 1993·Science·L A AaltonenS R Hamilton
May 7, 1993·Science·S N ThibodeauD Schaid
Jun 1, 1996·Japanese Journal of Cancer Research : Gann·Y AkiyamaY Yuasa
Sep 3, 1996·Proceedings of the National Academy of Sciences of the United States of America·J G HermanS B Baylin
Oct 29, 1997·Proceedings of the National Academy of Sciences of the United States of America·S OlschwangG Thomas
Jun 17, 1998·Proceedings of the National Academy of Sciences of the United States of America·J G HermanS B Baylin
Sep 11, 1998·Bone Marrow Transplantation·P EllerbroekE J Petersen
Jan 12, 1999·Experimental Cell Research·N Claij, H te Riele
Jan 15, 1999·British Journal of Cancer·D LazzereschiR Mariani-Costantini
Mar 13, 1999·International Journal of Cancer. Journal International Du Cancer·D M ParkinJ Ferlay
Jul 8, 1999·The American Journal of Pathology·K C HallingS M Powell
Sep 25, 1999·International Journal of Cancer. Journal International Du Cancer·H SuzukiK Imai
Nov 7, 1999·The American Journal of Pathology·M EstellerJ G Herman
Mar 24, 2000·Trends in Genetics : TIG·S B Baylin, J G Herman

❮ Previous
Next ❯

Citations

Dec 18, 2001·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·G R AndersonB M Brenner
Dec 13, 2005·Surgery Today·Shinya OdaYoshihiko Maehara
May 4, 2012·Clinical & Experimental Metastasis·J BischoffR Schneider-Stock
Oct 21, 2005·The Journal of Molecular Diagnostics : JMD·Julinor BacaniSteven Gallinger
Aug 16, 2003·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Susan C AbrahamTsung-Teh Wu
Feb 16, 2006·Molecular and Cellular Biology·Rachel A FreibergAmato J Giaccia
May 1, 2010·American Journal of Physiology. Gastrointestinal and Liver Physiology·Heather FrancisSharon DeMorrow
Jun 11, 2005·American Journal of Respiratory and Critical Care Medicine·Giovanna E CarpagnanoAngelo Paradiso
Dec 18, 2003·The Journal of Clinical Investigation·Haiwei H Guo, Lawrence A Loeb
May 31, 2008·Stem Cells·Francisco Javier Rodríguez-JiménezJosé-María Sánchez-Puelles
Jan 10, 2012·World Journal of Gastroenterology : WJG·Pu-Yuan WuYi-Mei Fan
May 30, 2013·World Journal of Gastroenterology : WJG·Marisa C AlvarezMarcelo L Ribeiro
Sep 12, 2013·Journal of Gastrointestinal Cancer·Meysam MoghbeliMohammad Reza Abbaszadegan
May 24, 2003·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·B CarvalhoR Seruca
Jul 13, 2007·European Journal of Gastroenterology & Hepatology·Masato SakuraiYoshihiko Maehara
May 26, 2007·Diagnostic Molecular Pathology : the American Journal of Surgical Pathology, Part B·Eva GömöriAndrás Matolcsy
Jul 30, 2015·Expert Review of Gastroenterology & Hepatology·Lei LiuJun Yu
Oct 4, 2005·Expert Opinion on Biological Therapy·Bela BodeyStuart E Siegel
Sep 1, 2009·Expert Opinion on Medical Diagnostics·Jan BornscheinPeter Malfertheiner
Apr 3, 2012·Digestive and Liver Disease : Official Journal of the Italian Society of Gastroenterology and the Italian Association for the Study of the Liver·Sumitaka YamanakaFlorin M Selaru
Nov 19, 2008·Seminars in Diagnostic Pathology·Fátima CarneiroRaquel Seruca
Oct 7, 2004·Environmental and Molecular Mutagenesis·Mark MeyersDavid A Boothman
Mar 25, 2006·International Journal of Cancer. Journal International Du Cancer·David ShibataStephen J Meltzer
Dec 20, 2005·Cancer·Fumiaki Sato, Stephen J Meltzer
May 28, 2010·Journal of Cellular Biochemistry·Kamaleshwar P SinghJames W Dumond
Feb 24, 2010·International Journal of Cancer. Journal International Du Cancer·Tamotsu SugaiKazuyuki Suzuki
Dec 25, 2004·Cancer Letters·Temduang LimpaiboonVajarabhongsa Bhudhisawasdi
Mar 8, 2005·Clinics in Laboratory Medicine·Adrian GologanAntonia R Sepulveda
Oct 24, 2014·Asian Pacific Journal of Cancer Prevention : APJCP·Jae-Ho Lee, Dae-Kwang Kim
Jun 20, 2015·BioMed Research International·Changlu GaoQifan Zhang
Jan 9, 2014·Radiation Research·Constantinos G Broustas, Howard B Lieberman
Feb 27, 2014·BMC Medicine·Tibor T GlantTibor A Rauch
Nov 7, 1999·The American Journal of Pathology·M EstellerJ G Herman

❮ Previous
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