Hyperosmolarity associated with diabetes insipidus alters hepatocyte structure and function but not survival after orthotopic liver transplantation in rats

Transplantation
S S FlormanC M Miller

Abstract

This study was designed to evaluate the effect of donor hyperosmolarity secondary to diabetes insipidus, an almost universal occurrence among brain-dead patients, on hepatic function. In vitro (isolated liver perfusion) and in vivo (hyaluronic acid and indocyanine green uptake, arterial ketone body ratio, orthotopic liver transplantation) experiments were conducted using Brattleboro rats, with hereditary hypothalamic diabetes insipidus, and Sprague-Dawley rats, with normal pituitary function. ATP content and recovery after cold preservation were measured during the perfusion. Cold-preserved livers from hyperosmolar rats were observed to have elevated hepatic enzyme release and decreased bile production compared with normosmolar controls. Moreover, in these livers, the recovery of ATP after cold preservation was completely absent. Transmission electron microscopy of liver biopsies collected from hyperosmolar rats demonstrated profound ultrastructural changes, particularly in the mitochondria, that were not evident in the biopsies from normosmolar rats. All the experimental groups demonstrated similar hyaluronic acid uptake, whereas indocyanine green uptake was markedly impaired in the hyperosmolar group, suggesting that hepatocy...Continue Reading

References

Sep 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·B A ScalettarC R Hackenbrock
May 1, 1988·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·A LanirM E Clouse
Jun 1, 1987·Critical Care Medicine·D H FiserR Woody
Jan 1, 1985·Cell and Tissue Research·J R FraserG B Ryan
Dec 1, 1973·Transplantation·S LeeM J Orloff
Sep 1, 1984·Neurology·K M Outwater, M A Rockoff
Apr 1, 1982·Journal of Biochemistry·W KamiikeY Kawashima
Aug 1, 1995·The International Journal of Biochemistry & Cell Biology·F MartínezM T Espinosa-García

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Citations

May 12, 2001·Liver Transplantation : Official Publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society·M Kukan, P S Haddad
Feb 23, 2007·The American Journal of Nursing·Kathleen M Z Peiffer
Nov 26, 2010·Transplantation Proceedings·E B RangelJ O Medina-Pestana
Oct 29, 2002·Anesthesia and Analgesia·Bruno JawanChao-Long Chen

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