Hyperosmotic Stimulus Down-regulates 1alpha, 25-dihydroxyvitamin D(3)-induced Osteoclastogenesis by Suppressing the RANKL Expression in a Co-culture System.

The Korean Journal of Physiology & Pharmacology : Official Journal of the Korean Physiological Society and the Korean Society of Pharmacology
Yu Shun TianSyng-Ill Lee

Abstract

The hyperosmotic stimulus is regarded as a mechanical factor for bone remodeling. However, whether the hyperosmotic stimulus affects 1alpha, 25-dihydroxyvitamin D(3) (1alpha,25(OH)(2)D(3))-induced osteoclastogenesis is not clear. In the present study, the effect of the hyperosmotic stimulus on 1alpha,25(OH)(2)D(3)-induced osteoclastogenesis was investigated in an osteoblast-preosteoclast co-culture system. Serial doses of sucrose were applied as a mechanical force. These hyperosmotic stimuli significantly evoked a reduced number of 1alpha,25(OH)(2)D(3)-induced tartrate-resistant acid phosphatase-positive multinucleated cells and 1alpha,25(OH)(2)D(3)-induced bone-resorbing pit area in a co-culture system. In osteoblastic cells, receptor activator of nuclear factor kappaB ligand (RANKL) and Runx2 expressions were down-regulated in response to 1alpha,25(OH)(2)D(3). Knockdown of Runx2 inhibited 1alpha,25(OH)(2)D(3)-induced RANKL expression in osteoblastic cells. Finally, the hyperosmotic stimulus induced the overexpression of TonEBP in osteoblastic cells. These results suggest that hyperosmolarity leads to the down-regulation of 1alpha,25(OH)(2)D(3)-induced osteoclastogenesis, suppressing Runx2 and RANKL expression due to the TonEB...Continue Reading

References

Jun 25, 1976·Science·S S MillerC D Arnaud
Dec 12, 1991·Biochimica Et Biophysica Acta·B Sarkadi, J C Parker
Jan 1, 1987·Annual Review of Medicine·R Marcus
Jan 1, 1981·Calcified Tissue International·G A Rodan, T J Martin
Sep 15, 1998·Biochemical and Biophysical Research Communications·J Klein-NulendE H Burger
Apr 21, 1999·Biochimica Et Biophysica Acta·R KitazawaS Maeda
Apr 25, 2000·American Journal of Physiology. Heart and Circulatory Physiology·X BaoJ A Frangos
May 4, 2000·American Journal of Physiology. Cell Physiology·N X ChenR L Duncan
Sep 1, 2000·Journal of Cellular Biochemistry·M ZayzafoonL R McCabe
Sep 1, 2000·Science·S L Teitelbaum
Apr 24, 2002·Developmental Cell·Gerard Karsenty, Erwin F Wagner
Jul 11, 2002·Oral Diseases·T Katagiri, N Takahashi
Aug 9, 2002·Molecular and Cellular Biology·Valérie GeoffroyPatrick Matthias
Sep 19, 2002·The Journal of Pathology·Sohei Kitazawa, Riko Kitazawa
May 16, 2003·Nature·William J BoyleDavid L Lacey
Aug 5, 2003·Nature Reviews. Genetics·Steven L Teitelbaum, F Patrick Ross
Oct 12, 2004·Biochemical and Biophysical Research Communications·Michitaka NotoyaHiromi Hagiwara
Mar 15, 2005·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Hiroshi TakatsunaKazuo Umezawa
Mar 23, 2005·Biochemical and Biophysical Research Communications·James G McGarryPatrick J Prendergast
Jun 15, 2006·The Journal of Biological Chemistry·Tsung-Ting TsaiMakarand V Risbud

❮ Previous
Next ❯

Citations

Mar 15, 2012·The Korean Journal of Physiology & Pharmacology : Official Journal of the Korean Physiological Society and the Korean Society of Pharmacology·Aran SonDong Min Shin
Oct 29, 2021·Journal of Biomedical Nanotechnology·Qingxuan WangChenchen Zhou

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.