Hyperosmotic stress and elevated pCO2 alter monoclonal antibody charge distribution and monosaccharide content

Biotechnology Progress
Albert E Schmelzer, W M Miller

Abstract

Medium osmolality increases with pCO2 at constant pH. Elevated pCO2 and osmolality inhibit hybridoma growth to similar extents in both serum-containing and serum-free media. The combination of osmolality and elevated pCO2 synergizes to negatively impact cell growth. IgG2a glycosylation by hybridoma cells was evaluated under elevated pCO2 (to 250 mmHg pCO2) and/or osmolality (to 476 mOsm/kg). IgG2a site occupancy did not change significantly under any of the conditions studied, which is consistent with the robust glycosylation of other antibodies produced under various environmental stresses. However, changes were observed in the IgG2a charge distribution. Changes in the isoelectric point (pI) were greater under hyperosmotic stress, increasing by 0.32 and 0.41 pH units at 435 mOsm/kg in serum-containing and serum-free medium, respectively. Hyperosmotic stress also resulted in a concomitant increase in the heterogeneity of the charge distribution. The mean pI in serum-containing medium decreased by 0.16 pH units at 250 mmHg pCO2 when osmolality was controlled at 320 mOsm/kg but increased by 0.20 pH units when the osmolality increased with pCO2 (195 mmHg pCO2-435 mOsm/kg). In serum-free medium, elevated pCO2 did not alter pI, rega...Continue Reading

Citations

Nov 6, 2004·Nature Biotechnology·Arthur J Chirino, Anthony Mire-Sluis
Sep 11, 2007·Journal of Pharmaceutical Sciences·Hongcheng LiuJoanne Sun
Mar 13, 2009·Biotechnology and Bioengineering·Zizhuo XingSteven S Lee
Feb 18, 2011·Biotechnology and Bioengineering·Michael J GramerPatrick H C van Berkel
Oct 15, 2013·Biotechnology Progress·Valerie Liu TsangThomas Ryll
Jan 1, 2010·Biotechnology Progress·Maria Elisa RodriguesRosário Oliveira
Oct 7, 2004·Current Opinion in Biotechnology·Dana C Andersen, Dorothea E Reilly
Apr 14, 2015·Biotechnology Progress·David BrühlmannHervé Broly
Oct 23, 2016·Biotechnology Progress·Hongcheng LiuAlyssa Neill
Dec 7, 2016·Biotechnology and Bioengineering·Zizhuo XingZheng Jian Li
Jan 1, 2019·Biotechnology and Bioengineering·Anh Nguyen DangInn H Yuk
Jan 20, 2019·Biotechnology and Bioengineering·David BrühlmannMartin Jordan
Jun 9, 2019·Journal of Industrial Microbiology & Biotechnology·Bernhard SissolakKarola Vorauer-Uhl
Apr 26, 2017·Applied Microbiology and Biotechnology·Shiwei MiaoWen-Song Tan
Jun 11, 2019·Biotechnology Progress·Jakob WallnerKarola Vorauer-Uhl
Feb 24, 2007·Biotechnology and Bioengineering·Shinya TakumaJames M Piret
Aug 9, 2018·Bioprocess and Biosystems Engineering·Rubin JiangSen Xu
Dec 29, 2020·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Jun-Jie YuanHai-Bin Wang

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