Hyperoxia disrupts pulmonary epithelial barrier in newborn rats via the deterioration of occludin and ZO-1.

Respiratory Research
Kai YouXindong Xue

Abstract

Prolonged exposure to hyperoxia in neonates can cause hyperoxic acute lung injury (HALI), which is characterized by increased pulmonary permeability and diffuse infiltration of various inflammatory cells. Disruption of the epithelial barrier may lead to altered pulmonary permeability and maintenance of barrier properties requires intact epithelial tight junctions (TJs). However, in neonatal animals, relatively little is known about how the TJ proteins are expressed in the pulmonary epithelium, including whether expression of TJ proteins is regulated in response to hyperoxia exposure. This study determines whether changes in tight junctions play an important role in disruption of the pulmonary epithelial barrier during hyperoxic acute lung injury. Newborn rats, randomly divided into two groups, were exposed to hyperoxia (95% oxygen) or normoxia for 1-7 days, and the severity of lung injury was assessed; location and expression of key tight junction protein occludin and ZO-1 were examined by immunofluorescence staining and immunobloting; messenger RNA in lung tissue was studied by RT-PCR; transmission electron microscopy study was performed for the detection of tight junction morphology. We found that different durations of hyper...Continue Reading

References

Feb 21, 1998·The American Journal of Physiology·T A Appel
Dec 9, 2000·American Journal of Respiratory and Critical Care Medicine·H DengR L Auten
Apr 3, 2001·American Journal of Respiratory and Critical Care Medicine·E D Crandall, M A Matthay
May 24, 2001·American Journal of Respiratory and Critical Care Medicine·L B Ware, M A Matthay
Jun 13, 2001·American Journal of Respiratory and Critical Care Medicine·A H Jobe, E Bancalari
Jul 13, 2002·American Journal of Physiology. Lung Cellular and Molecular Physiology·Suzy A A Comhair, Serpil C Erzurum
Dec 12, 2002·Progress in Biophysics and Molecular Biology·L González-MariscalB E Jaramillo
Apr 2, 2003·Seminars in Neonatology : SN·Eduardo BancalariIlene R S Sosenko
Apr 2, 2003·Seminars in Neonatology : SN·Jacqueline J Coalson
Jul 11, 2003·Free Radical Biology & Medicine·Robert P JankovA Keith Tanswell
Jun 12, 2004·The Journal of Pediatrics·Vincent C SmithDouglas K Richardson
Sep 7, 2004·American Journal of Respiratory and Critical Care Medicine·Man YiA Keith Tanswell
Apr 12, 2005·Respiratory Research·Karmene AhamedAlexandra Henrion-Caude
Jun 14, 2005·American Journal of Physiology. Lung Cellular and Molecular Physiology·Jörg ReutershanKlaus Ley
Jun 22, 2005·Proceedings of the National Academy of Sciences of the United States of America·Yasuhiro GonHugh Rosen
Apr 18, 2006·Pediatric Pulmonology·Lex W Doyle, UNKNOWN Victorian Infant Collaborative Study Group
Jul 25, 2006·Seminars in Perinatology·Peter J Anderson, Lex W Doyle
Jan 2, 2007·Current Opinion in Critical Care·William A Altemeier, Scott E Sinclair
Aug 19, 2007·American Journal of Physiology. Lung Cellular and Molecular Physiology·Tianbo LiHans G Folkesson
Nov 1, 2007·Respiratory Research·Emanuela Mazzon, Salvatore Cuzzocrea
Sep 2, 2008·American Journal of Respiratory Cell and Molecular Biology·Mingjie WangShaojie Yue
Apr 25, 2009·Pediatric Research·Richard L Auten, Jonathan M Davis
Dec 26, 2009·American Journal of Physiology. Lung Cellular and Molecular Physiology·Neil R AggarwalLandon S King
Mar 3, 2010·Molecular and Cellular Endocrinology·Helen ChasiotisScott P Kelly
Dec 28, 2010·American Journal of Physiology. Lung Cellular and Molecular Physiology·Tricia I LozonWilliam A Altemeier
May 3, 2011·American Journal of Respiratory Cell and Molecular Biology·Gustavo Matute-BelloUNKNOWN Acute Lung Injury in Animals Study Group
May 4, 2011·The Journal of Cell Biology·David R RaleighJerrold R Turner

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Citations

Dec 17, 2014·British Journal of Anaesthesia·W Habre, F Peták
Nov 20, 2013·Experimental Lung Research·Qiu-ping LiuFa-guang Jin
May 31, 2014·Pediatrics and Neonatology·Ying-Chun LuHueng-Chuen Fan
May 9, 2015·Seminars in Cell & Developmental Biology·Barbara SchlingmannMichael Koval
Sep 30, 2014·Biochemical and Biophysical Research Communications·Ying FengHuifang Cao
Oct 11, 2014·Reproduction : the Official Journal of the Society for the Study of Fertility·Daniel HerrChristine Wulff
Feb 15, 2015·American Journal of Physiology. Lung Cellular and Molecular Physiology·Ana HouXindong Xue
Apr 26, 2017·International Journal of Molecular Medicine·Li YaoXindong Xue
Jun 15, 2018·International Journal of Molecular Medicine·Mengyun LiXindong Xue
Jun 13, 2015·Anesthesiology·Joshua A EnglertRebecca M Baron
Apr 27, 2018·Environmental Science and Pollution Research International·Joshua B LewisPaul R Reynolds
Oct 21, 2016·International Journal of Environmental Research and Public Health·Joshua B LewisPaul R Reynolds
Mar 2, 2017·Histochemistry and Cell Biology·Andrea PeterStefan Hippenstiel
Sep 28, 2017·International Journal of Molecular Medicine·Haiping YangXindong Xue
May 31, 2018·Molecular Medicine Reports·Zhixian TangChunfa Xie
Jun 8, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Tee Yee SimDaud Ahmad Israf
Sep 30, 2014·Physiological Reports·Jonathan DaviesMichael R Blackburn
May 7, 2021·Environmental Science and Pollution Research International·Chia-Hua LinLung-Chun Wang

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Methods Mentioned

BETA
bronchoalveolar lavage
dissection
PCR
transmission electron microscopy
Bronchoalveolar

Software Mentioned

ChemiImager 5500
Fluor Chen
SPSS
Primer Premier

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