Hyperoxia exposure impairs nephrogenesis in the neonatal rat: role of HIF-1α

PloS One
Constantin R PopescuAnne Monique Nuyt

Abstract

Preterm neonates are exposed at birth to high oxygen concentrations relative to the intrauterine environment. We have previously shown in a rat model that a hyperoxic insult results in a reduced nephron number in adulthood. Therefore, the aim of this study was to determine the effects of transient neonatal hyperoxia exposure on nephrogenesis. Sprague-Dawley rat pups were raised in 80% O2 or room air from P3 to P10. Pups (n = 12/group, 6 males and 6 females) were sacrificed at P5 (during active nephrogenesis) and at P10 (after the completion of nephrogenesis). Hyperoxia exposure resulted in a significant reduction in both nephrogenic zone width and glomerular diameter at P5, and a significantly increased apoptotic cell count; however, nephron number at P10 was not affected. HIF-1α expression in the developing kidney was significantly reduced following hyperoxia exposure. Systemic administration of the HIF-1α stabilizer dimethyloxalylglycine (DMOG) resulted in enhanced expression of HIF-1α and improved nephrogenesis: kidneys from hyperoxia-exposed pups treated with DMOG exhibited a nephrogenic zone width and glomerular diameter similar to room-air controls. These findings demonstrate that neonatal hyperoxia exposure results in im...Continue Reading

References

Feb 1, 1992·The American Journal of Physiology·D Dreher, T Rochat
Mar 15, 1997·Developmental Biology·A Tufro-McReddieR A Gomez
Apr 24, 2001·Current Opinion in Obstetrics & Gynecology·O D Saugstad
Jun 1, 2002·Journal of the American Society of Nephrology : JASN·Virginie MattotPeter Carmeliet
Jun 19, 2002·European Journal of Obstetrics, Gynecology, and Reproductive Biology·George D GeorgesonAranka László
Feb 4, 2003·Pediatrics·Lex W DoyleRuth Morley
Mar 6, 2003·The Journal of Clinical Investigation·Vera EreminaSusan E Quaggin
Mar 18, 2003·The Journal of Pediatrics·Máximo VentoJosé Viña
Mar 28, 2003·Journal of the American Society of Nephrology : JASN·Paul B FreeburgDale R Abrahamson
Jun 5, 2003·Nature Medicine·Christopher W Pugh, Peter J Ratcliffe
Mar 23, 2004·Annals of the New York Academy of Sciences·Alessandra Pagano, Constance Barazzone-Argiroffo
Jul 17, 2004·Pediatric and Developmental Pathology : the Official Journal of the Society for Pediatric Pathology and the Paediatric Pathology Society·Maria M RodríguezGastón E Zilleruelo
Mar 23, 2005·Pediatric Nephrology : Journal of the International Pediatric Nephrology Association·Juan Rodríguez-SorianoAlfredo Vallo
Oct 6, 2005·Journal of Pediatric Gastroenterology and Nutrition·Andrea KovácsTamás Decsi
Dec 24, 2005·Kidney International·W M BernhardtK-U Eckardt
Aug 4, 2006·American Journal of Physiology. Renal Physiology·Ningjun LiPin-Lan Li
Jan 9, 2007·American Journal of Physiology. Lung Cellular and Molecular Physiology·Vivek BalasubramaniamSteven H Abman
Mar 9, 2007·Journal of the American Society of Nephrology : JASN·Ichiro KojimaMasaomi Nangaku
Nov 1, 2007·American Journal of Perinatology·Silvia IacobelliJean Bernard Gouyon
Feb 9, 2008·Cell Death and Differentiation·G-H Fong, K Takeda
Oct 15, 2008·Hypertension·Catherine YzydorczykAnne Monique Nuyt
Dec 6, 2008·Proceedings of the National Academy of Sciences of the United States of America·Jonathan E SearsBela Anand-Apte
Jan 2, 2009·Journal of the American Society of Nephrology : JASN·Lakshman Gunaratnam, Joseph V Bonventre
May 13, 2009·Current Opinion in Nephrology and Hypertension·Anne Monique Nuyt, Barbara T Alexander
Sep 10, 2009·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Young Rim SongKi Young Na
Sep 18, 2009·American Journal of Physiology. Renal Physiology·Lina GubhajuM Jane Black
Dec 17, 2009·Pediatric Nephrology : Journal of the International Pediatric Nephrology Association·Mandy G Keijzer-VeenBert J van der Heijden
May 11, 2010·Seminars in Fetal & Neonatal Medicine·Jonathan M Davis, Richard L Auten
Jul 1, 2010·Journal of Immunotoxicology·Ashwini GoreLin L Mantell
Jul 6, 2010·Seminars in Fetal & Neonatal Medicine·Angela M ParkEmin Maltepe

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Citations

Apr 26, 2015·Pediatrics and Neonatology·Hsin-Hui Wang
Mar 30, 2016·Current Opinion in Nephrology and Hypertension·Bjoern BuchholzKai-Uwe Eckardt
Jul 19, 2016·Journal of Hypertension·Megan R SutherlandMary Jane Black
Feb 17, 2017·Pediatrics International : Official Journal of the Japan Pediatric Society·Mayu NakagawaToshiaki Shimizu
Dec 28, 2017·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·Vasantha H S KumarRita M Ryan
Aug 17, 2016·Physiological Reports·Megan R SutherlandAnne Monique Nuyt
Oct 28, 2017·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Simone WurmMiriam C Banas
Aug 20, 2019·Renal Failure·Hsiu-Chu Chou, Chung-Ming Chen
Feb 6, 2019·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Jasmine MohrMiguel A Alejandre Alcazar
Dec 6, 2019·Pediatric Research·Jennifer R CharltonKevin M Bennett
Apr 12, 2020·Journal of Perinatology : Official Journal of the California Perinatal Association·Matthew W HarerKimberly J Reidy
Jul 6, 2018·International Journal of Molecular Sciences·Jochen HinkelbeinAndreas Hohn
Sep 30, 2014·Physiological Reports·Jonathan DaviesMichael R Blackburn
Nov 16, 2020·International Immunopharmacology·Arun KumarVishal Aggarwal
Dec 22, 2020·Journal of Cardiothoracic and Vascular Anesthesia·Jinyoung BaeKaram Nam

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Methods Mentioned

BETA
electrophoresis

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Image Pro Plus
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