Hyperoxia induces glutamine-fuelled anaplerosis in retinal Müller cells.

Nature Communications
Charandeep SinghJonathan E Sears

Abstract

Although supplemental oxygen is required to promote survival of severely premature infants, hyperoxia is simultaneously harmful to premature developing tissues such as in the retina. Here we report the effect of hyperoxia on central carbon metabolism in primary mouse Müller glial cells and a human Müller glia cell line (M10-M1 cells). We found decreased flux from glycolysis entering the tricarboxylic acid cycle in Müller cells accompanied by increased glutamine consumption in response to hyperoxia. In hyperoxia, anaplerotic catabolism of glutamine by Müller cells increased ammonium release two-fold. Hyperoxia induces glutamine-fueled anaplerosis that reverses basal Müller cell metabolism from production to consumption of glutamine.

References

Mar 1, 1978·Pediatric Research·M L Batshaw, S W Bursilow
Aug 1, 1996·Trends in Neurosciences·E Newman, A Reichenbach
Jul 2, 2002·American Journal of Respiratory Cell and Molecular Biology·Sharon A McGrath-Morrow, Jennifer Stahl
Jul 15, 2006·Progress in Retinal and Eye Research·Andreas BringmannAndreas Reichenbach
Feb 19, 2008·The Journal of Biological Chemistry·Huafeng ZhangGregg L Semenza
Aug 19, 2008·Investigative Ophthalmology & Visual Science·Lois E H Smith
Dec 17, 2008·Brain Research·Rie HosoiOsamu Inoue
Dec 31, 2008·Neurochemistry International·Andreas BringmannAndreas Reichenbach
Feb 17, 2009·Methods in Enzymology·Charleen T ChuRobert S B Clark
May 7, 2009·Journal of Neurochemistry·Dinesh K DeelchandPierre-Gilles Henry
Feb 4, 2010·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Anant B PatelGraeme F Mason
Nov 23, 2011·Proceedings of the National Academy of Sciences of the United States of America·David R WiseCraig B Thompson
Mar 16, 2012·Bioinformatics·Pierre MillardJean-Charles Portais
Aug 27, 2013·Cell Metabolism·Katrien De BockPeter Carmeliet
Sep 4, 2013·The Journal of Clinical Investigation·Christopher T HensleyRalph J DeBerardinis
Dec 5, 2013·Biochemical and Biophysical Research Communications·Zuheng MaAnneli Björklund
May 7, 2014·The Journal of Biological Chemistry·Mulchand S PatelFrank Jordan
Oct 15, 2014·Proceedings of the National Academy of Sciences of the United States of America·Ken J LindsayJames B Hurley
Jul 1, 2017·The EMBO Journal·Boa KimZoltan Arany
Feb 9, 2018·Arteriosclerosis, Thrombosis, and Vascular Biology·Cui MaElizabeth R Jacobs
Feb 12, 2019·Annual Review of Physiology·Xuri LiPeter Carmeliet

❮ Previous
Next ❯

Citations

Jul 14, 2020·Frontiers in Cell and Developmental Biology·George HoppeJonathan E Sears

❮ Previous
Next ❯

Methods Mentioned

BETA
deamidation
dissections
protein assay

Software Mentioned

IsoCor
Mass Hunter
Metabolite Detector
ImageJ
Mitophagy
MiNA

Related Concepts

Related Feeds

Astrocytes

Astrocytes are glial cells that support the blood-brain barrier, facilitate neurotransmission, provide nutrients to neurons, and help repair damaged nervous tissues. Here is the latest research.

Related Papers

Gazette des hôpitaux civils et militaires de l'Empire ottoman
P COUMEL
Rivista di medicina aeronautica
A SCANO
Rivista di medicina aeronautica
A SCANO
Journal of Neurosurgery
M Ross Bullock
Journal of Neurosurgery
Gaylan L Rockswold, Sarah B Rockswold
© 2021 Meta ULC. All rights reserved