Hyperoxia induces the differentiated neuronal phenotype of PC12 cells by producing reactive oxygen species

Biochemical and Biophysical Research Communications
S KatohT Suzuki

Abstract

Neurite extension of PC12 cells induced by nerve growth factor (NGF) is a well-known model of neuronal differentiation. In this study, the incubation of PC12 cells in a 50% O2 atmosphere (hyperoxia) caused neurite extension. In these cells, amounts of differentiation-marker proteins, tyrosine hydroxylase and neurofilament M increased. The effects of hyperoxia were inhibited by ascorbic acid or N-acetyl-cysteine, antioxidant reagents, suggesting the involvement of reactive oxygen species (ROS). In support of this, artificial generation of free radicals induced the same effects as hyperoxia. In these cells, total phosphorylation of cellular proteins was enhanced similar to NGF-treated cells. These results suggest that hyperoxia enhances the signal for neuronal differentiation by producing ROS, resulting in the induction of the differentiated neuronal phenotype of PC12 cells.

Citations

Dec 2, 2005·Neuropsychopharmacology : Official Publication of the American College of Neuropsychopharmacology·Ruth R JamesonTheodore A Slotkin
Mar 18, 2010·The Journal of Biological Chemistry·Karen A M KennedyShawn S C Li
Apr 1, 2008·The Journal of Biological Chemistry·Rayudu GopalakrishnaThomas H McNeill
Aug 30, 2013·Journal of Biomedical Science·Claudio MuscariCarlo Guarnieri
Apr 6, 2013·Biology Direct·Katarína VaškovičováJan Brábek
Nov 6, 2002·Environmental Health Perspectives·Dan QiaoTheodore A Slotkin
Jan 9, 2010·Environmental Health Perspectives·Christina M PowersTheodore A Slotkin
Sep 7, 2007·Environmental Health Perspectives·Theodore A SlotkinFrederic J Seidler
Jun 19, 2008·Environmental Health Perspectives·Theodore A SlotkinFrederic J Seidler
Jan 5, 2000·Proceedings of the National Academy of Sciences of the United States of America·O YermolaievaT Hoshi
Nov 26, 2014·Sensors·Fatima AlZahra'a AlatraktchiWinnie E Svendsen
Mar 3, 2011·Neuroscience Letters·Elena A Ostrakhovitch, Oleg A Semenikhin
Jun 17, 2015·Molecular and Cellular Neurosciences·Vidya ChandrasekaranPamela J Lein
Nov 4, 2008·Brain Research Bulletin·T Leon LassiterTheodore A Slotkin
Nov 4, 2010·Developmental Dynamics : an Official Publication of the American Association of Anatomists·J A PargaJ L Labandeira-Garcia
Jun 21, 2005·Toxicology and Applied Pharmacology·Dan QiaoTheodore A Slotkin
Feb 15, 2005·Brain Research. Developmental Brain Research·Yael Abreu-VillaçaTheodore A Slotkin
Mar 29, 2006·Neuroscience Letters·Maryam FaizBerta González
Jun 18, 2016·Frontiers in Cellular Neuroscience·Roberta AccettaMariarosaria Santillo
Jan 22, 2011·Progress in Neurobiology·Helena L A VieiraAlessandro Vercelli
Mar 29, 2013·Neurochemistry International·Angélica CoyoyJulio Morán
Feb 24, 2001·Breast Cancer Research and Treatment·T A Slotkin, F J Seidler
Jun 8, 2007·Journal of Neurophysiology·Dominic P D'AgostinoJay B Dean
Sep 29, 2011·Cellular and Molecular Life Sciences : CMLS·Karen A M KennedyShawn S-C Li
Jun 6, 2009·European Biophysics Journal : EBJ·G MaulucciM De Spirito
Jan 15, 2014·Journal of Physiology and Biochemistry·Monika SobczakLudmila Zylinska
Feb 5, 2021·Journal of Neuroscience Research·Laura A Richards, Christopher M Schonhoff
Jul 20, 2001·Archives of Gerontology and Geriatrics·K OraveczI Zs -Nagy

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.