Hyperoxic injury decreases alveolar epithelial cell expression of vascular endothelial growth factor (VEGF) in neonatal rabbit lung

American Journal of Respiratory Cell and Molecular Biology
W M ManiscalcoR M Ryan

Abstract

Normal neonatal lung growth requires a substantial increase in microvascular endothelial cells. Oxygen injury to neonatal lung destroys endothelial cells and alters the normal process of alveolarization, including development of the microvasculature. The mechanisms that regulate lung alveolar capillary growth and development are not known. Vascular endothelial growth factor (VEGF) is a specific mitogen for endothelial cells that is often expressed by epithelial cells in close proximity to capillary beds. VEGF expression is induced by hypoxia and may be inhibited by hyperoxia. We examined the cell-specific expression of VEGF during normal postnatal lung development and the effects of hyperoxic lung injury on VEGF mRNA and protein in vivo. Normal newborn rabbits between 1 day and 5 wk of age had VEGF transcripts located mainly in alveolar epithelial cells, with little or no VEGF mRNA noted in smooth muscle or endothelial cells. A subpopulation of freshly isolated, normal type II cells, but not mesenchymal cells, expressed VEGF mRNA. Newborn rabbits exposed to 100% oxygen for 4 days had no change in VEGF mRNA abundance, transcript location, or immunostaining. Animals exposed to 100% oxygen for an average of 9 days had an 80% decre...Continue Reading

Citations

Feb 13, 2001·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Y S NgP A D'Amore
Sep 21, 2002·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Darren B TaichmanH Scott Baldwin
Jan 19, 1999·Critical Care Clinics·T E Albertson, G P Marelich
Feb 26, 2004·Paediatric Respiratory Reviews·Robert P Jankov, A Keith Tanswell
Feb 11, 2012·Pediatric Research·Beatriz AlmarioIlene R S Sosenko
Nov 15, 2002·Journal of Anatomy·Alison A Hislop
Jan 10, 2004·Antioxidants & Redox Signaling·Maria Lyn Quintos-AlaghebandMargaret A Schwarz
Apr 16, 2011·Current Opinion in Pediatrics·Jason Gien, John P Kinsella
Feb 5, 2009·Respirology : Official Journal of the Asian Pacific Society of Respirology·Joo Hwa ShinJung Yeon Shim
Aug 16, 2005·American Journal of Physiology. Lung Cellular and Molecular Physiology·Richard L AutenMary H Whorton
Aug 17, 2010·American Journal of Physiology. Lung Cellular and Molecular Physiology·Jen-Ruey TangSteven H Abman
Sep 8, 2009·American Journal of Physiology. Lung Cellular and Molecular Physiology·Hideshi FujinagaSteven H Abman
Feb 23, 2010·American Journal of Physiology. Lung Cellular and Molecular Physiology·J Davin MillerLawrence S Prince
May 6, 1999·American Journal of Respiratory and Critical Care Medicine·P LassusS Andersson
Nov 24, 2001·American Journal of Respiratory and Critical Care Medicine·D R ThickettA B Millar
Jun 11, 2005·American Journal of Respiratory and Critical Care Medicine·Faruqa LadhaBernard Thébaud
Feb 3, 2007·American Journal of Respiratory and Critical Care Medicine·Bernard Thébaud, Steven H Abman
Apr 28, 2007·American Journal of Respiratory and Critical Care Medicine·David E SimcockStuart J Hirst
Sep 8, 2007·American Journal of Respiratory and Critical Care Medicine·Jason GienSteven H Abman
Jun 17, 2008·American Journal of Respiratory and Critical Care Medicine·David E SimcockStuart J Hirst
Jul 3, 2008·American Journal of Respiratory and Critical Care Medicine·Steven H Abman
Sep 8, 2009·American Journal of Respiratory and Critical Care Medicine·Vivek Balasubramaniam, David A Ingram
Dec 31, 1998·American Journal of Respiratory Cell and Molecular Biology·M J AcarreguiJ M Snyder
Apr 27, 2000·American Journal of Respiratory Cell and Molecular Biology·C Barazzone, C W White
Feb 22, 2005·American Journal of Respiratory Cell and Molecular Biology·Sharon A McGrath-MorrowRubin M Tuder
Feb 18, 2006·American Journal of Respiratory Cell and Molecular Biology·Emmanuel LopezPierre-Henri Jarreau
Feb 27, 2009·American Journal of Respiratory Cell and Molecular Biology·Trent E TippleLynette K Rogers
Sep 10, 2009·American Journal of Respiratory Cell and Molecular Biology·Arul VadivelBernard Thébaud
Jun 11, 2011·American Journal of Respiratory Cell and Molecular Biology·Deepthi AlapatiShu Wu
Feb 5, 2013·American Journal of Respiratory Cell and Molecular Biology·Robin H SteinhornSteven H Abman
Sep 16, 2004·Clinical Evidence·David Creery, Angelo Mikrogianakis
Nov 29, 2007·Pediatric Research·Americo E EsquibiesLloyd G Cantley

❮ Previous
Next ❯

Related Concepts

Related Feeds

American Thoracic Association Journals

Discover the latest respiratory research published by the journals from the American Thoracic Society.