Hyperoxic regulation of lung heme oxygenase in neonatal rats

Pediatric Research
Phyllis A DenneryV Shokoohi

Abstract

In the neonatal lung, hyperoxic exposure is associated with induction of various genes and critical antioxidants. Heme oxygenase, specifically the HO-1 isoenzyme, is regulated in oxidant stress and may also serve to limit oxidative damage. However, it is not known whether neonatal lung HO-1 is regulated in hyperoxia specifically and, if so, what type of regulation occurs. Therefore, we attempted to answer these questions using newly born (< 12 h) Wistar rats exposed to hyperoxia for 3 d. Neonatal rat lungs were evaluated daily for total HO activity, immunoreactive HO-1 protein, and steady state levels of HO-1 mRNA and compared with air-exposed controls. In neonatal rats, we noted an increased lung HO activity after 3 d of hyperoxic exposure. Additionally, evaluation of HO activity after immunoprecipitation of HO-1 protein suggested that HO-1 contributed most of the increase in lung total HO activity observed in hyperoxia. Nonetheless, we did not see a significant difference in immunoreactive HO-1 protein in neonatal lungs after 3 d of hyperoxic exposure, although we did so on d 2. Also, in contrast with previous reports, we did not detect any significant differences in steady state levels of HO-1 mRNA on any day of hyperoxic ex...Continue Reading

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Citations

Dec 25, 2002·Free Radical Biology & Medicine·Phyllis A DenneryGuang Yang
Sep 17, 2002·Antioxidants & Redox Signaling·Stefan W Ryter, Augustine M K Choi
Jan 9, 2010·Annual Review of Pharmacology and Toxicology·Raffaella GozzelinoMiguel P Soares
Nov 9, 2000·American Journal of Respiratory and Critical Care Medicine·S LimK F Chung
Apr 16, 1998·The Journal of Clinical Investigation·P A DenneryK D Poss
Jan 1, 2003·Pediatric Research·Phyllis A DenneryPamela A Rodgers
May 9, 2003·Pediatric Research·Doron KreiserPhyllis A Dennery
Oct 25, 2008·Pediatric Research·Sacha Kassovska-BratinovaPhyllis A Dennery
Mar 10, 2006·The Journal of Steroid Biochemistry and Molecular Biology·Maximiliano CellaAna M Franchi
Sep 28, 2015·Respiratory Research·Kelsa GabehartAzzeddine Dakhama
Aug 16, 2005·Journal of Korean Medical Science·Youngsoo HanJie-Young Song
May 7, 2003·Journal of Perinatology : Official Journal of the California Perinatal Association·Joshua D MilnerAyman El-Mohandes
Jun 28, 2016·American Journal of Physiology. Lung Cellular and Molecular Physiology·Hayato GoPhyllis A Dennery
Jul 26, 2015·Cellular & Molecular Biology Letters·Farhana ZahirAdel M Abuzenadah
Jul 13, 2000·American Journal of Physiology. Lung Cellular and Molecular Physiology·P J LeeA M Choi
Oct 11, 2011·American Journal of Physiology. Lung Cellular and Molecular Physiology·Wesley M KonsavageJeffrey S Shenberger
Feb 9, 2000·American Journal of Physiology. Lung Cellular and Molecular Physiology·G YangP A Dennery
Jun 3, 2000·American Journal of Physiology. Lung Cellular and Molecular Physiology·Y H WengP A Dennery

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