Hyperphosphorylation of Tau and filopodial retraction following microinjection of protein kinase C catalytic subunits

Journal of Neuroscience Research
C M Cressman, T B Shea

Abstract

Limited proteolysis of protein kinase C (PKC) by calcium-activated proteolysis cleaves the regulatory and catalytic subunits of PKC, generating a free, constitutively activated kinase ("PKM") that, unlike the intact parent enzyme, is not calcium-dependent, and is not restricted to the plasma membrane. These latter properties leave open the possibility that PKM may have access to, and may therefore phosphorylate, substrates normally unavailable to intact PKC. We examined the potential involvement of such aberrant phosphorylation in certain aspects of the neurodegeneration accompanying Alzheimer's disease by microinjecting PKC and PKM, along with a rhodamine-conjugated dextran tracer, into undifferentiated NB2a/d1 mouse neuroblastoma cells. After 4 hr, cultures were fixed and processed for immunofluorescence with monoclonal antibodies (PHF-1, ALZ-50, Tau-1, AT8) directed against tau in various phosphorylation states followed by fluorescein-conjugated secondary antibodies. Microinjected cells were localized via co-injected rhodamine-conjugated dextran tracer under rhodamine illumination, after which antibody immunoreactivity was examined under fluorescein illumination. Microdensitometric analyses indicated that microinjection of P...Continue Reading

References

Sep 15, 1992·European Journal of Biochemistry·A AzziC Hensey
Feb 1, 1991·Cell Biology International Reports·T B Shea, M L Beermann
May 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·S PontremoliB L Horecker
May 2, 1986·Science·B L WolozinP Davies
Jun 15, 1987·The Biochemical Journal·S YoungS Stabel
Jun 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·S PontremoliB L Horecker
Jun 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·M GoedertV M Lee

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Citations

Mar 31, 1999·Cell Motility and the Cytoskeleton·C Jung, T B Shea
Apr 1, 2000·Cell Motility and the Cytoskeleton·J T YabeT B Shea
Oct 16, 2003·Brain Research. Molecular Brain Research·Fatma J EkinciThomas B Shea
Jun 1, 1997·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·J J Boyce, T B Shea
May 6, 1998·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·F J Ekinci, T B Shea
Jul 17, 1998·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·T B Shea, C M Cressman
Jul 24, 2013·Cellular Signalling·C A Heckman, H K Plummer

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